Erythrocyte binding antigen (EBA-175) of Plasmodium falciparum : improved genotype determination by nested polymerase chain reaction

We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)-175 gene which amplify specifically the two alleles by polymerase chain reaction (PCR) and by nested PCR. This approach provides a specific, sensitive and rapid method for genotype determination in a large numb...

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Bibliographic Details
Published in:Tropical medicine & international health 2001-10, Vol.6 (10), p.767-769
Main Authors: TOURE, Fousseyni S, MAVOUNGOU, Elie, MEZUI ME NDONG, Jérome, TSHIPAMBA, Paul, DELORON, Philippe
Format: Article
Language:English
Subjects:
Animals
Antigens, Protozoan - genetics
Biological and medical sciences
Carrier Proteins - genetics
DNA Primers
Genotype
Human protozoal diseases
Humans
Infectious diseases
Malaria
Malaria, Falciparum - epidemiology
Medical sciences
Parasitic diseases
Plasmodium falciparum - genetics
Polymerase Chain Reaction - standards
Protozoal diseases
Protozoan Proteins - genetics
Sensitivity and Specificity
Tropical medicine
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Description
Summary:We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)-175 gene which amplify specifically the two alleles by polymerase chain reaction (PCR) and by nested PCR. This approach provides a specific, sensitive and rapid method for genotype determination in a large number of Plasmodium falciparum field isolates.
ISSN:1360-2276
1365-3156