A transcript encoding a proteasome beta-subunit and a zinc finger protein in Trypanosoma brucei brucei

During the screening of a Trypanosoma brucei brucei ( T. b. brucei) cDNA library constructed from bloodstream form mRNA, we identified a 2.3 kb cDNA encoding a proteasome beta subunit (ORF1) and a putative zinc finger protein (ORF2). Northern blot analysis indicated the presence of a digenic transcr...

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Bibliographic Details
Published in:Gene 2000-09, Vol.255 (1), p.43-50
Main Authors: Radwanska, Magdalena, Couvreur, Bernard, Dumont, Nathalie, Pays, Annette, Vanhamme, Luc, Pays, Etienne
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cysteine Endopeptidases - genetics
DNA, Complementary - chemistry
DNA, Complementary - genetics
Molecular Sequence Data
Multienzyme Complexes - genetics
Open Reading Frames
Orf1 gene
Orf2 gene
Phylogenetic analysis
Phylogeny
Polycistronic transcription
Proteasome Endopeptidase Complex
proteasomes
Protein Subunits
RNA, Messenger - genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Transcription, Genetic
Trypanosoma brucei
Trypanosoma brucei brucei - genetics
Zinc Fingers - genetics
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Summary:During the screening of a Trypanosoma brucei brucei ( T. b. brucei) cDNA library constructed from bloodstream form mRNA, we identified a 2.3 kb cDNA encoding a proteasome beta subunit (ORF1) and a putative zinc finger protein (ORF2). Northern blot analysis indicated the presence of a digenic transcript as well as the two individual messengers in both procyclic and bloodstream forms of the parasite. Southern blot analysis showed the relevant locus to be unique. ORF1 encoded a 22.7 kDa protein sharing over 50% identity with the eukaryotic PRCE (aka beta5) proteasome beta subunit. This protein contained a beta amino acid signature and residues involved in the catalytic activity. Further phylogenetic analysis indicated that this subunit as well as those from other kinetoplastids could be confidentially assigned to extant eukaryotic subfamilies such as beta1, beta2, and beta5. ORF2 encoded a 14.6 kDa putative zinc finger protein containing five repeats of a CCHC motif commonly present in retroviral nucleocapsid proteins as well as proteins involved in vertebrate embryogenesis.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(00)00306-1