Sequencing of oligosaccharides by collision-induced dissociation matrix-assisted laser desorption/ionization mass spectrometry

A study of the collision‐induced dissociation post‐source decay (PSD) spectra of free oligosaccharides is presented. These spectra, when obtained with helium as collision gas, show 1,5X fragments containing the reducing end sugar. The presence of these fragments permits Y ions and, consequently, B a...

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Bibliographic Details
Published in:Journal of mass spectrometry. 2000-08, Vol.35 (8), p.1042-1048
Main Authors: Spina, Emanuela, Cozzolino, Rosaria, Ryan, Elizabeth, Garozzo, Domenico
Format: Article
Language:English
Subjects:
Carbohydrate Sequence
Helium
matrix-assisted laser desorption/ionization
Molecular Sequence Data
oligosaccharides
Oligosaccharides - chemistry
post-source decay
Sequence Analysis - methods
sequencing
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
sugars
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Summary:A study of the collision‐induced dissociation post‐source decay (PSD) spectra of free oligosaccharides is presented. These spectra, when obtained with helium as collision gas, show 1,5X fragments containing the reducing end sugar. The presence of these fragments permits Y ions and, consequently, B and C peaks to be identified. This is a common behaviour from which it has been possible to delineate a general method for the easy assignment of the peaks in PSD spectra of underivatized neutral sugars, allowing the sequence of a real unknown to be obtained. Copyright © 2000 John Wiley & Sons, Ltd.
ISSN:1076-5174
1096-9888
DOI:10.1002/1096-9888(200008)35:8<1042::AID-JMS33>3.0.CO;2-Y