Multiple Transcripts of MUC3: Evidence for Two Genes, MUC3A and MUC3B

The MUC3 gene encodes a transmembrane mucin-type glycoprotein. A number of consistent single nucleotide changes were observed in different MUC3 cDNAs from a single individual, suggesting the presence of at least three different transcripts per individual. This transcript heterogeneity is due both to...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-09, Vol.275 (3), p.916-923
Main Authors: Pratt, Wendy S., Crawley, Suzanne, Hicks, Jim, Ho, Jenny, Nash, Matthew, Kim, Young S., Gum, James R., Swallow, Dallas M.
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Base Sequence
Caco-2 Cells
Cloning, Molecular
Exons - genetics
Gene Expression
Genes, Duplicate - genetics
Genetic Variation - genetics
Humans
Intestines - embryology
Intestines - metabolism
Introns - genetics
Molecular Sequence Data
MUC3 gene
Mucin-3
Mucins - chemistry
Mucins - genetics
Polymorphism, Genetic - genetics
Repetitive Sequences, Amino Acid - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Messenger - genetics
Sequence Alignment
Sequence Analysis, DNA
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Summary:The MUC3 gene encodes a transmembrane mucin-type glycoprotein. A number of consistent single nucleotide changes were observed in different MUC3 cDNAs from a single individual, suggesting the presence of at least three different transcripts per individual. This transcript heterogeneity is due both to the existence of a second copy of the MUC3 gene and to allelic changes. Sequencing of the second MUC3 shows that it has the same C-terminal domain and intron/exon structure as the previously described MUC3. The tandem repeat domain has the same amino acid consensus sequence but shows more substitutions. The unique exonic sequences range from 94 to 100% identity at the nucleotide level and correspondingly few amino acid changes have been identified. The introns show around 95% identity. We propose to name this second gene MUC3B, MUC3A being reserved for the first MUC3 gene. MUC3B, like MUC3A, is expressed in intestine and Caco-2 cells.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.3406