Automated determination of carboxyhemoglobin contents in autopsy materials using head-space gas chromatography/mass spectrometry

To establish a method for the routine analysis of carboxyhemoglobin (COHb) in autopsy materials including those which have undergone postmortem changes, e.g. thermo-coagulation, putrifaction and contamination, an automated head-space gas chromatography/mass spectrometry (GC/MS) analysis was utilized...

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Bibliographic Details
Published in:Forensic science international 2000-09, Vol.113 (1), p.375-379
Main Authors: Oritani, Shigeki, Zhu, Bao-Li, Ishida, Kaori, Shimotouge, Keiko, Quan, Li, Fujita, Masaki Q, Maeda, Hitoshi
Format: Article
Language:English
Subjects:
Automation
Autopsies
Autopsy
Autopsy - instrumentation
Autopsy - methods
Biological and medical sciences
Blood Coagulation
Bone marrow
Bone Marrow - chemistry
Butanol
Carbon monoxide
Carboxyhemoglobin
Carboxyhemoglobin - analysis
Carboxyhemoglobin - metabolism
Chemical and industrial products toxicology. Toxic occupational diseases
Chromatography
Contamination
Forensic toxicology
Gas chromatography
Gas Chromatography-Mass Spectrometry - instrumentation
Gas Chromatography-Mass Spectrometry - methods
Gas chromatography/mass spectrometry
Gas sampling
Gas, fumes
Headspace
Hematoma
Hemoglobin
Hemoglobins - analysis
Hot Temperature
Humans
Mass spectrometry
Mass spectroscopy
Medical sciences
Methods
Octanol
Oximetry
Postmortem Changes
Potassium
Potassium ferricyanide
Sensitivity and Specificity
Thermocoagulated blood
Toxicology
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Summary:To establish a method for the routine analysis of carboxyhemoglobin (COHb) in autopsy materials including those which have undergone postmortem changes, e.g. thermo-coagulation, putrifaction and contamination, an automated head-space gas chromatography/mass spectrometry (GC/MS) analysis was utilized. The procedure consisted of preparation of the sample in a vial and a carbon monoxide (CO) saturated sample, for estimation of hemoglobin content, in another vial, the addition of n-octanol, potassium ferricyanide and an internal standard ( t-butanol), GC separation and determination of CO using a GC/MS system equipped with an automated head-space gas sampler. The method was practical not only with the blood and bone marrow aspirates to confirm the findings on the CO-oximeter system, but also with the thermo-coagulated and putrified blood.
ISSN:0379-0738
1872-6283
DOI:10.1016/S0379-0738(00)00227-9