Nanoporous biocapsules for the encapsulation of insulinoma cells: biotransport and biocompatibility considerations

This study investigates whether nanoporous micromachined biocapsules, with uniform membrane pore sizes of 24.5-nm, can be used to encapsulate insulin-secreting cells in vitro. This approach to cell encapsulation is based on microfabrication technology whereby immunoisolation membranes are bulk and s...

Full description

Saved in:
Bibliographic Details
Published in:IEEE transactions on biomedical engineering 2001-11, Vol.48 (11), p.1335-1341
Main Authors: Leoni, L., Desai, T.A.
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials
Biological and medical sciences
Biomedical Engineering
Biomembranes
Capsules
Chemical technology
Chemistry
Diabetes. Impaired glucose tolerance
Diffusion
Diffusion Chambers, Culture
Encapsulation
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Glucose - metabolism
Hormones. Endocrine system
Immune system
In vitro
Insulin
Insulin - metabolism
Insulin Secretion
Insulinoma - metabolism
Islets of Langerhans Transplantation
Kinetics
Management. Various non-drug treatments. Langerhans islet grafts
Materials Testing
Medical sciences
Mice
Microarchitecture
Nanoporous materials
Pancreatic Neoplasms - metabolism
Particle Size
Pharmacology. Drug treatments
Sugar
Surface Properties
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study investigates whether nanoporous micromachined biocapsules, with uniform membrane pore sizes of 24.5-nm, can be used to encapsulate insulin-secreting cells in vitro. This approach to cell encapsulation is based on microfabrication technology whereby immunoisolation membranes are bulk and surface micromachined to present uniform and well-controlled pore sizes as small as 10 nm, tailored surface chemistries, and precise microarchitectures. This study evaluates the behavior of insulinoma cells with micromachined membranes, the effect of matrix configurations within the biocapsule on cell behavior, as well as insulin and glucose transport through the biocapsule membranes.
ISSN:0018-9294
1558-2531
DOI:10.1109/10.959329