Superficially porous silica microspheres for fast high-performance liquid chromatography of macromolecules

Very fast reversed-phase separations of biomacromolecules are performed using columns made with superficially porous silica microsphere column packings (“Poroshell”). These column packings consist of ultra-pure “biofriendly” silica microspheres composed of solid cores and thin outer shells with unif...

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Bibliographic Details
Published in:Journal of Chromatography A 2000-08, Vol.890 (1), p.3-13
Main Authors: Kirkland, J.J., Truszkowski, F.A., Dilks, C.H., Engel, G.S.
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Fundamental and applied biological sciences. Psychology
Microspheres
Nucleic acids
Nucleic Acids - analysis
Particle Size
Proteins
Proteins - analysis
Silicon Dioxide - chemistry
Triton X-114
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Summary:Very fast reversed-phase separations of biomacromolecules are performed using columns made with superficially porous silica microsphere column packings (“Poroshell”). These column packings consist of ultra-pure “biofriendly” silica microspheres composed of solid cores and thin outer shells with uniform pores. The excellent kinetic properties of these new column packings allow stable, high-resolution gradient chromatography of polypeptides, proteins, nucleic acids, DNA fragments, etc. in a fraction of the time required for conventional separations. Contrasted with
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)00392-7