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Isolation and Characterization of Myrianthus holstii Lectin, a Potent HIV-1 Inhibitory Protein from the Plant Myrianthus holstii

Aqueous extracts from the African plant Myrianthus holstii potently inhibited the infection of the T-lymphoblastoid cell line, CEM-SS, by human immunodeficiency virus-1RF (HIV-1RF). The active constituent, M. holstii lectin (MHL), was purified by LH-20 column chromatography and reversed phase HPLC....

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2000-08, Vol.63 (8), p.1170-1174
Main Authors: Charan, Romila D, Munro, Murray H. G, O'Keefe, Barry R, Sowder, Raymond C, McKee, Tawnya C, Currens, Michael J, Pannell, Lewis K, Boyd, Michael R
Format: Article
Language:English
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Summary:Aqueous extracts from the African plant Myrianthus holstii potently inhibited the infection of the T-lymphoblastoid cell line, CEM-SS, by human immunodeficiency virus-1RF (HIV-1RF). The active constituent, M. holstii lectin (MHL), was purified by LH-20 column chromatography and reversed phase HPLC. MHL, a 9284-Da cysteine-rich protein, was characterized by amino acid analysis, N-terminal sequencing, ESIMS, and matrix-assisted laser-desorption ionizationāˆ’time-of-flight mass spectrometry. Pure MHL had anti-HIV activity, with an EC50 value of 150 nM. Delaying the addition of MHL for up to 8 h after initial exposure of CEM-SS cells to virus did not result in loss of the antiviral activity; however, if addition of the compound was delayed for 16 h or more, there was a marked decrease in the antiviral activity. MHL bound to a virus-free, soluble form of the viral envelope protein gp120 but did not inhibit the subsequent binding to a cell-free, soluble form of the cellular receptor CD4.
ISSN:0163-3864
1520-6025
DOI:10.1021/np000039h