Long Term Depression in the CA1 Field Is Associated with a Transient Decrease in Pre- and Postsynaptic PKC Substrate Phosphorylation

Induction of homosynaptic long term depression (LTD) in the CA1 field of the hippocampus is thought to require activation of N -methyl- d -aspartate receptors, an elevation of postsynaptic Ca 2+ levels, and a subsequent increase in phosphatase activity. To investigate the spatial and temporal change...

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Bibliographic Details
Published in:The Journal of biological chemistry 2000-09, Vol.275 (37), p.28682-28687
Main Authors: Ramakers, G M, Heinen, K, Gispen, W H, de Graan, P N
Format: Article
Language:English
Subjects:
Animals
Calmodulin-Binding Proteins - metabolism
GAP-43 Protein - metabolism
Hippocampus - physiology
In Vitro Techniques
Nerve Tissue Proteins - metabolism
Neurogranin
Phosphorylation
Protein Kinase C - metabolism
Rats
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Summary:Induction of homosynaptic long term depression (LTD) in the CA1 field of the hippocampus is thought to require activation of N -methyl- d -aspartate receptors, an elevation of postsynaptic Ca 2+ levels, and a subsequent increase in phosphatase activity. To investigate the spatial and temporal changes in protein phosphatase activity following LTD induction, we determined the in situ phosphorylation state of a pre- (GAP-43/B-50) and postsynaptic (RC3) protein kinase C substrate during N -methyl- d -aspartate receptor-dependent LTD in the CA1 field of rat hippocampal slices. We show that LTD is associated with a transient (
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M003068200