Cloning of Schistosoma japonicum 14-3-3 epsilon (Sj14-3-3ε), a new member of the 14-3-3 family of proteins from schistosomes

A new member of the 14-3-3 protein family from Schistosoma japonicum has been identified. Phylogenetic analysis showed that this member belongs to the epsilon subfamily of the 14-3-3 proteins, and it is therefore named Sj14-3-3ε. Consistent with the findings for the previously reported S. japonicum...

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Bibliographic Details
Published in:International journal for parasitology 2000-08, Vol.30 (9), p.991-994
Main Authors: Zhang, Yaobi, Bickle, Quentin D., Taylor, Martin G.
Format: Article
Language:English
Subjects:
14-3-3 protein
14-3-3 Proteins
Amino Acid Sequence
Animals
Base Sequence
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Cloning, Molecular
Epsilon isoform
Fundamental and applied biological sciences. Psychology
Invertebrates
Molecular Sequence Data
Molecular Weight
Nemathelminthia. Plathelmintha
Phylogeny
Physiology. Development
Polymerase Chain Reaction - veterinary
Protein Isoforms
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
RNA - chemistry
RNA - isolation & purification
Schistosoma japonicum
Schistosoma japonicum - chemistry
Schistosoma japonicum - classification
Schistosoma japonicum - genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Stage-specific expression
Tyrosine 3-Monooxygenase - chemistry
Tyrosine 3-Monooxygenase - genetics
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Summary:A new member of the 14-3-3 protein family from Schistosoma japonicum has been identified. Phylogenetic analysis showed that this member belongs to the epsilon subfamily of the 14-3-3 proteins, and it is therefore named Sj14-3-3ε. Consistent with the findings for the previously reported S. japonicum 14-3-3 protein (Sj14-3-3), Southern analysis suggested the presence of more than one gene, and/or introns or allelic polymorphism in this epsilon isoform. By RT–PCR, Sj14-3-3ε was shown to be stage-specifically transcribed, being abundant in adults, present in sporocysts but absent in cercariae. Furthermore, mRNA of the epsilon isoform seemed to be much less abundant in the sporocyst stage, compared with Sj14-3-3. This suggests varying requirements of the different 14-3-3 isoforms at different stages of the life cycle.
ISSN:0020-7519
1879-0135
DOI:10.1016/S0020-7519(00)00086-2