A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion

A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye...

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Bibliographic Details
Published in:Journal of controlled release 2001-11, Vol.77 (1), p.1-6
Main Authors: Kockisch, Sandra, Rees, Gareth D, Young, Simon A, Tsibouklis, John, Smart, John D
Format: Article
Language:English
Subjects:
Adhesiveness
Bioadhesion
Biological and medical sciences
Chitosan
General pharmacology
Humans
Medical sciences
Mouth Mucosa - metabolism
Oral cavity
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Poly(acrylic acid)
Polymers - metabolism
Staining and Labeling
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Summary:A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.
ISSN:0168-3659
1873-4995
DOI:10.1016/S0168-3659(01)00444-8