A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion

A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye...

Full description

Saved in:
Bibliographic Details
Published in:Journal of controlled release 2001-11, Vol.77 (1), p.1-6
Main Authors: Kockisch, Sandra, Rees, Gareth D, Young, Simon A, Tsibouklis, John, Smart, John D
Format: Article
Language:English
Subjects:
Adhesiveness
Bioadhesion
Biological and medical sciences
Chitosan
General pharmacology
Humans
Medical sciences
Mouth Mucosa - metabolism
Oral cavity
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Poly(acrylic acid)
Polymers - metabolism
Staining and Labeling
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c391t-278569b1f1174754c1c913a1b5c4c2c48d0cd7916e2784340a1f687be0983d0b3
cites cdi_FETCH-LOGICAL-c391t-278569b1f1174754c1c913a1b5c4c2c48d0cd7916e2784340a1f687be0983d0b3
container_end_page 6
container_issue 1
container_start_page 1
container_title Journal of controlled release
container_volume 77
creator Kockisch, Sandra
Rees, Gareth D
Young, Simon A
Tsibouklis, John
Smart, John D
description A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.
doi_str_mv 10.1016/S0168-3659(01)00444-8
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72251130</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168365901004448</els_id><sourcerecordid>72251130</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-278569b1f1174754c1c913a1b5c4c2c48d0cd7916e2784340a1f687be0983d0b3</originalsourceid><addsrcrecordid>eNqFkU1P3DAQhi1EBQvtTwD5AoJDwBPbSXxCKwS00ko9QA89WY4zYY2SeLETJP493g_BkYsteZ6ZefWYkBNgV8CguH5MR5XxQqoLBpeMCSGyao_MoCp5JpSS-2T2iRySoxhfGGOSi_KAHEIqqFyKGfk_p40LaMcsjsYNbnimPY5L39DRU3wz3WRGpOMSaT9Zb5olRucH6lu68t17jyHSNviemtcJ_RTTsLhKj4n5SX60pov4a3cfk3_3d0-3v7PF34c_t_NFZrmCMcvLShaqhhagFKUUFqwCbqCWVtjciqphtikVFJhIwQUz0BZVWSNTFW9YzY_J-XbuKvgUIo66d9Fi15lhnUiXeS4BOEug3II2-BgDtnoVXG_Cuwam1071xqleC9MM9MaprlLf6W7BVPfYfHXtJCbgbAeYaE3XBjNYF784kdYXMk_czZbDpOPNYdDROhwsbn9AN959E-UDPgKTIw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72251130</pqid></control><display><type>article</type><title>A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion</title><source>ScienceDirect Journals</source><creator>Kockisch, Sandra ; Rees, Gareth D ; Young, Simon A ; Tsibouklis, John ; Smart, John D</creator><creatorcontrib>Kockisch, Sandra ; Rees, Gareth D ; Young, Simon A ; Tsibouklis, John ; Smart, John D</creatorcontrib><description>A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.</description><identifier>ISSN: 0168-3659</identifier><identifier>EISSN: 1873-4995</identifier><identifier>DOI: 10.1016/S0168-3659(01)00444-8</identifier><identifier>PMID: 11689254</identifier><identifier>CODEN: JCREEC</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adhesiveness ; Bioadhesion ; Biological and medical sciences ; Chitosan ; General pharmacology ; Humans ; Medical sciences ; Mouth Mucosa - metabolism ; Oral cavity ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Poly(acrylic acid) ; Polymers - metabolism ; Staining and Labeling</subject><ispartof>Journal of controlled release, 2001-11, Vol.77 (1), p.1-6</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-278569b1f1174754c1c913a1b5c4c2c48d0cd7916e2784340a1f687be0983d0b3</citedby><cites>FETCH-LOGICAL-c391t-278569b1f1174754c1c913a1b5c4c2c48d0cd7916e2784340a1f687be0983d0b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=14113652$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11689254$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kockisch, Sandra</creatorcontrib><creatorcontrib>Rees, Gareth D</creatorcontrib><creatorcontrib>Young, Simon A</creatorcontrib><creatorcontrib>Tsibouklis, John</creatorcontrib><creatorcontrib>Smart, John D</creatorcontrib><title>A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion</title><title>Journal of controlled release</title><addtitle>J Control Release</addtitle><description>A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.</description><subject>Adhesiveness</subject><subject>Bioadhesion</subject><subject>Biological and medical sciences</subject><subject>Chitosan</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mouth Mucosa - metabolism</subject><subject>Oral cavity</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Poly(acrylic acid)</subject><subject>Polymers - metabolism</subject><subject>Staining and Labeling</subject><issn>0168-3659</issn><issn>1873-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkU1P3DAQhi1EBQvtTwD5AoJDwBPbSXxCKwS00ko9QA89WY4zYY2SeLETJP493g_BkYsteZ6ZefWYkBNgV8CguH5MR5XxQqoLBpeMCSGyao_MoCp5JpSS-2T2iRySoxhfGGOSi_KAHEIqqFyKGfk_p40LaMcsjsYNbnimPY5L39DRU3wz3WRGpOMSaT9Zb5olRucH6lu68t17jyHSNviemtcJ_RTTsLhKj4n5SX60pov4a3cfk3_3d0-3v7PF34c_t_NFZrmCMcvLShaqhhagFKUUFqwCbqCWVtjciqphtikVFJhIwQUz0BZVWSNTFW9YzY_J-XbuKvgUIo66d9Fi15lhnUiXeS4BOEug3II2-BgDtnoVXG_Cuwam1071xqleC9MM9MaprlLf6W7BVPfYfHXtJCbgbAeYaE3XBjNYF784kdYXMk_czZbDpOPNYdDROhwsbn9AN959E-UDPgKTIw</recordid><startdate>20011109</startdate><enddate>20011109</enddate><creator>Kockisch, Sandra</creator><creator>Rees, Gareth D</creator><creator>Young, Simon A</creator><creator>Tsibouklis, John</creator><creator>Smart, John D</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011109</creationdate><title>A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion</title><author>Kockisch, Sandra ; Rees, Gareth D ; Young, Simon A ; Tsibouklis, John ; Smart, John D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-278569b1f1174754c1c913a1b5c4c2c48d0cd7916e2784340a1f687be0983d0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adhesiveness</topic><topic>Bioadhesion</topic><topic>Biological and medical sciences</topic><topic>Chitosan</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mouth Mucosa - metabolism</topic><topic>Oral cavity</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Poly(acrylic acid)</topic><topic>Polymers - metabolism</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kockisch, Sandra</creatorcontrib><creatorcontrib>Rees, Gareth D</creatorcontrib><creatorcontrib>Young, Simon A</creatorcontrib><creatorcontrib>Tsibouklis, John</creatorcontrib><creatorcontrib>Smart, John D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of controlled release</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kockisch, Sandra</au><au>Rees, Gareth D</au><au>Young, Simon A</au><au>Tsibouklis, John</au><au>Smart, John D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion</atitle><jtitle>Journal of controlled release</jtitle><addtitle>J Control Release</addtitle><date>2001-11-09</date><risdate>2001</risdate><volume>77</volume><issue>1</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>0168-3659</issn><eissn>1873-4995</eissn><coden>JCREEC</coden><abstract>A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11689254</pmid><doi>10.1016/S0168-3659(01)00444-8</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0168-3659
ispartof Journal of controlled release, 2001-11, Vol.77 (1), p.1-6
issn 0168-3659
1873-4995
language eng
recordid cdi_proquest_miscellaneous_72251130
source ScienceDirect Journals
subjects Adhesiveness
Bioadhesion
Biological and medical sciences
Chitosan
General pharmacology
Humans
Medical sciences
Mouth Mucosa - metabolism
Oral cavity
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Poly(acrylic acid)
Polymers - metabolism
Staining and Labeling
title A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T06%3A41%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20direct-staining%20method%20to%20evaluate%20the%20mucoadhesion%20of%20polymers%20from%20aqueous%20dispersion&rft.jtitle=Journal%20of%20controlled%20release&rft.au=Kockisch,%20Sandra&rft.date=2001-11-09&rft.volume=77&rft.issue=1&rft.spage=1&rft.epage=6&rft.pages=1-6&rft.issn=0168-3659&rft.eissn=1873-4995&rft.coden=JCREEC&rft_id=info:doi/10.1016/S0168-3659(01)00444-8&rft_dat=%3Cproquest_cross%3E72251130%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c391t-278569b1f1174754c1c913a1b5c4c2c48d0cd7916e2784340a1f687be0983d0b3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=72251130&rft_id=info:pmid/11689254&rfr_iscdi=true