FR167653, a potent suppressant of interleukin-1 and tumor necrosis factor-α production, ameliorates colonic lesions in experimentally induced acute colitis

• Published in: Journal of gastroenterology and hepatology 2001-10, Vol.16 (10), p.1105-1111
• Main Authors: Blandino, Ivan I Pacheco, Otaka, Michiro, Jin, Mario, Komatsu, Koga, Odashima, Masaru, Konishi, Noriaki, Sato, Toshihiro, Kato, Sayuri, Watanabe, Sumio
• Format: Article
• Language: English
• Subjects: Acetic Acid; Acute Disease; Analysis of Variance; Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Biological and medical sciences; Colitis - prevention & control; cytokine suppressant; Digestive system; Disease Models, Animal; experimental colitis; Interleukin-1 - antagonists & inhibitors; Interleukin-1 - biosynthesis; Interleukin-1 - blood; interleukin-β; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Molecular Structure; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Pyrazoles - chemistry; Pyrazoles - pharmacology; Pyridines - chemistry; Pyridines - pharmacology; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - biosynthesis; tumor necrosis factor-α
• ISSN: 0815-9319; 1440-1746
• DOI: 10.1046/j.1440-1746.2001.02584.x

Interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha) are believed to play a significant role in the pathogenesis of inflammatory bowel disease (IBD). Interleukin-1 and TNF-alpha possess overlapping and synergetic activities inducing the production in cascade of other cytokines, adhesion molecules, arachidonic acid metabolites, as well as activating immune and non-immune cells. FR167653 (C24H18FN5O2-H2SO4-H2O) is a newly synthesized organic compound with a potent inhibitory effect on IL-1beta and TNF-alpha production. We hypothesized that the suppression of IL-1 and TNF-alpha induced by FR167653 could effectively attenuate experimentally induced colonic damage. Colonic lesions were induced in male Sprague-Dawley rats (250-300 g) by intrarectal instillation of 4% acetic acid. The effect of FR167653 administration at 1.0, 1.5, 2.5 mg/kg per 6 h subcutaneously on acetic acid-induced colonic damage was assessed. The lesion area, microscopic findings, colonic and serum levels of TNF-alpha and IL-1beta were also evaluated. Treatment with FR167653 at 1.5 and 2.5 mg/kg per 6 h was able to ameliorate the gross macroscopic appearance of colonic lesions significantly, as well as ameliorate the lesion area induced by acetic acid. Colonic mucosal TNF-alpha and IL-1beta levels of rats treated with FR167653 showed significant decrease in a dose-dependent fashion compared with the control group. In the same manner, serum TNF-alpha of rats treated with FR167653 was significantly lower than that of respective controls. Subcutaneous administration of FR167653 was able to ameliorate the acute changes induced by acetic acid instillation in a dose-dependent manner. This is the first report to evaluate the dual inhibition of the production of IL-1 and TNF-alpha, offered by FR167653, in acute experimental colitis. Further studies are necessary to evaluate FR167653's efficacy and safety on long-term conditions.