Microstructured glass chip for ion-channel electrophysiology

We present a technique by which it is possible to produce a planar sensor for ion channel electrophysiology from glass substrates. Apertures with diameters in the low micrometer to submicrometer range are achieved by irradiation of a glass chip with a single heavy ion and subsequent wet track etchin...

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Bibliographic Details
Published in:Physical review. E, Statistical, nonlinear, and soft matter physics Statistical, nonlinear, and soft matter physics, 2001-10, Vol.64 (4 Pt 1), p.040901-040901
Main Authors: Fertig, N, Meyer, C, Blick, R H, Trautmann, C, Behrends, J C
Format: Article
Language:English
Subjects:
Electrolytes
Electrophysiology - methods
Glass
Gramicidin - chemistry
Ion Channels - chemistry
Ion Channels - physiology
Ions
Lipid Bilayers - chemistry
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Summary:We present a technique by which it is possible to produce a planar sensor for ion channel electrophysiology from glass substrates. Apertures with diameters in the low micrometer to submicrometer range are achieved by irradiation of a glass chip with a single heavy ion and subsequent wet track etching. The function of the device is demonstrated by recordings of single channel currents mediated by the model ion channel gramicidin A in lipid bilayers spanning the micromachined aperture.
ISSN:1539-3755
DOI:10.1103/physreve.64.040901