Aquaporin 4: lack of mRNA expression in the rat regenerating muscle fiber under denervation

The recently identified water channel aquaporin 4 is a major component of the orthogonal arrays observed with freeze-fracture electron microscopy. We examined the expression of aquaporin 4 mRNA and protein in rat regenerating muscle under innervated and denervated conditions. We found decreased sarc...

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Bibliographic Details
Published in:Neuroscience letters 2000-09, Vol.291 (2), p.93-96
Main Authors: Jimi, Takahiro, Wakayama, Yoshihiro, Murahashi, Makoto, Shibuya, Seiji, Inoue, Masahiko, Hara, Hajime, Matsuzaki, Yoko, Uemura, Norie
Format: Article
Language:English
Subjects:
Animals
Aquaporin 4
Aquaporins - biosynthesis
Aquaporins - genetics
Biological and medical sciences
Denervation
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Male
mRNA
Muscle Denervation
Muscle Fibers, Skeletal - physiology
Muscle regeneration
Muscle, Skeletal - innervation
Muscle, Skeletal - physiology
Muscular dystrophy
Nerve supply
Orthogonal array
Rats
Rats, Wistar
Regeneration - genetics
Regeneration - physiology
RNA, Messenger - biosynthesis
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
water channels
Water-Electrolyte Balance - physiology
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Summary:The recently identified water channel aquaporin 4 is a major component of the orthogonal arrays observed with freeze-fracture electron microscopy. We examined the expression of aquaporin 4 mRNA and protein in rat regenerating muscle under innervated and denervated conditions. We found decreased sarcolemmal immunostaining of aquaporin 4 in denervated regenerating muscle as opposed to innervated muscle. Quantitative reverse transcription-polymerase chain reaction revealed that aquaporin 4 mRNA was expressed in the innervated regenerating muscle; whereas it was not expressed in denervated muscle. Thus, lack of aquaporin 4 protein may be due to lack of aquaporin 4 mRNA in the denervated regenerating muscle. We conclude that the nerve supply influences expression of aquaporin 4 at the mRNA level in regenerating muscle.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(00)01382-3