Vacuolar-type H+ -ATPase distribution in unstimulated and acetylcholine-activated isolated human eccrine sweat glands

The presence and cellular distribution of subunits of the V1 sector of the vacuolar-type H+ -ATPase (V-ATPase) was investigated in isolated human eccrine sweat glands. In every instance, V-ATPase was located in the cytoplasm and apical membranes of the luminal cells of the reabsorptive duct segment....

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Bibliographic Details
Published in:Journal of molecular histology 2000-07, Vol.32 (7), p.409-413
Main Authors: Bovell, D L, Clunes, M T, Roussa, E, Burry, J, Elder, H Y
Format: Article
Language:English
Subjects:
Acetylcholine
Acetylcholine - pharmacology
Animals
Antibodies, Blocking - pharmacology
Cellular biology
Eccrine Glands - drug effects
Eccrine Glands - enzymology
Eccrine Glands - metabolism
Enzyme Activation - drug effects
Equidae - immunology
Humans
Immunoenzyme Techniques
In Vitro Techniques
Proteins
Proton-Translocating ATPases - metabolism
Vacuoles - enzymology
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Summary:The presence and cellular distribution of subunits of the V1 sector of the vacuolar-type H+ -ATPase (V-ATPase) was investigated in isolated human eccrine sweat glands. In every instance, V-ATPase was located in the cytoplasm and apical membranes of the luminal cells of the reabsorptive duct segment. In the secretory coil, both diffuse and perinuclear staining was demonstrated in the secretory cells, with additional expression at the apical and basolateral membranes and on the intercellular canaliculi. There was no detectable difference in V-ATPase expression as a result of prior application of 100 microM acetylcholine.
ISSN:0018-2214
1567-2379
1567-2387
1573-6865
DOI:10.1023/A:1004087120735