Lymphangiogenesis Quantification Using Quantitative PCR and Breast Cancer as a Model

The detection of lymphangiogenesis (formation of new lymphatics) has previously been difficult to measure, primarily due to the lack of specific markers for lymphatic endothelium. Using conventional PCR (polymerase chain reaction), DNA sequencing, plasmid synthesis, and real-time quantitative PCR (R...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-11, Vol.288 (4), p.1043-1046
Main Authors: Cunnick, G.H., Jiang, W.G., Gomez, K.F., Mansel, R.E.
Format: Article
Language:English
Subjects:
Biomarkers - analysis
breast cancer
Breast Neoplasms - genetics
Breast Neoplasms - immunology
Breast Neoplasms - pathology
Cloning, Molecular
Endothelium, Vascular - growth & development
Endothelium, Vascular - metabolism
Female
Glycoproteins - genetics
Humans
Lymph Nodes - growth & development
Lymph Nodes - metabolism
Lymph Nodes - pathology
lymphangiogenesis
Lymphatic Metastasis - immunology
Lymphatic Metastasis - pathology
LYVE-1
Plasmids - genetics
Polymerase Chain Reaction - methods
real-time quantitative polymerase chain reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sensitivity and Specificity
Vesicular Transport Proteins
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Summary:The detection of lymphangiogenesis (formation of new lymphatics) has previously been difficult to measure, primarily due to the lack of specific markers for lymphatic endothelium. Using conventional PCR (polymerase chain reaction), DNA sequencing, plasmid synthesis, and real-time quantitative PCR (RTQPCR), we report a new approach to enable the measurement of lymphangiogenesis using LYVE-1, a novel, specific lymphatic marker in breast cancer tissue. By using a Scorpion-based probe system with the RTQPCR analyser, a highly sensitive and specific detection and quantitation of LYVE-1 was possible. It was found that lymphangiogenesis occurred in all breast specimens and that higher levels were found in tumours which had spread to the lymph nodes.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5869