Interleukin-12 prevents diaphragm muscle deterioration in a septic animal model

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2001-11, Vol.130 (4), p.653-663
• Main Authors: Nakahata, Eri, Shindoh, Yuriko, Takayama, Tetsuro, Shindoh, Chiyohiko
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphatases - metabolism; Animals; ATPase; Cytokine; Diaphragm - enzymology; Diaphragm - pathology; Disease Models, Animal; Electrophysiology; Endotoxin; Endotoxins - pharmacology; Free radicals; Immunohistochemistry; Interleukin-12 - pharmacology; Kinetics; Muscle Contraction; Muscle Fibers, Skeletal - metabolism; Muscles - enzymology; Muscles - pathology; NADP - metabolism; NADPH Dehydrogenase - pharmacology; NADPH diaphorase; Nitric oxide; Nitric Oxide - metabolism; Rats; Rats, Wistar; Sepsis - metabolism; Skeletal muscle; Superoxide; Time Factors
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/S1095-6433(01)00396-8

The effects of an intravenous injection of Interleukin-12 (IL-12) after endotoxin administration and without endotoxin administration on diaphragm muscle were studied using Wistar rats. Three treatment groups, namely a control (Saline+endotoxin) group, an IL-12+endotoxin group and an IL-12 only group were studied. E. coli endotoxin (30 mg/kg) was injected intraperitoneally 5 min after Saline or IL-12 (0.25 μg) injection. In the control group, the force–frequency curves, twitch tension (TT) and slope during contraction time (TT/CT) were significantly lower at 4 h than those at 0 h due to endotoxin ( P