Neuralized functions as an E3 ubiquitin ligase during Drosophila development

The Notch pathway is a widely studied means of intercellular signaling responsible for the determination of cell fate, cell differentiation, and boundary formation (reviewed in [1, 2]). The main effectors of this pathway, Notch (N) and Delta (Dl), have been shown to function as a receptor and ligand...

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Bibliographic Details
Published in:Current biology 2001-10, Vol.11 (21), p.1675-1679
Main Authors: Yeh, Edward, Dermer, Matt, Commisso, Cosimo, Zhou, Lily, McGlade, C.Jane, Boulianne, Gabrielle L
Format: Article
Language:English
Subjects:
Animals
Cell Compartmentation
Cell Membrane - metabolism
Delta (Dl) gene
Drosophila
Drosophila - embryology
Drosophila Proteins
E3 ubiquitin ligase
Ligases - metabolism
Membrane Proteins - metabolism
Mutation
Nerve Tissue Proteins - metabolism
Neuralized protein
notch (N) gene
RING finger protein
RING finger proteins
Sequence Deletion
Ubiquitin-Protein Ligases
Zinc Fingers
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Summary:The Notch pathway is a widely studied means of intercellular signaling responsible for the determination of cell fate, cell differentiation, and boundary formation (reviewed in [1, 2]). The main effectors of this pathway, Notch (N) and Delta (Dl), have been shown to function as a receptor and ligand, respectively. Genetic and phenotypic studies suggest that Neuralized (Neu), a RING finger protein, also plays a role within the N-Dl pathway, although its biochemical function is unknown. Here, we show that Neu is required at the plasma membrane for functional activity and that its RING finger domain acts as an E3 ubiquitin ligase. These data suggest that the role of Neu is to target components of the N-Dl pathway for ubiquitination, allowing for propagation and/or regulation of the signal.
ISSN:0960-9822
1879-0445
DOI:10.1016/S0960-9822(01)00527-9