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Characterization of an arabinogalactan-protein isolated from pressed juice of Echinacea purpurea by precipitation with the β-glucosyl Yariv reagent
An arabinogalactan-protein (AGP) from pressed juice of Echinacea purpurea herb was isolated from a high molecular weight fraction by precipitation with the β-glucosyl Yariv reagent, followed by gel-permeation chromatography. It revealed characteristic features of other AGPs: i.e., a high amount of p...
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| Published in: | Carbohydrate research 2000-08, Vol.327 (4), p.497-504 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c361t-8ceb97162bd6423cc19f6cd38a41b1353899c1429b7ed590a9dffcbe8479f2743 |
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| cites | cdi_FETCH-LOGICAL-c361t-8ceb97162bd6423cc19f6cd38a41b1353899c1429b7ed590a9dffcbe8479f2743 |
| container_end_page | 504 |
| container_issue | 4 |
| container_start_page | 497 |
| container_title | Carbohydrate research |
| container_volume | 327 |
| creator | Classen, Birgit Witthohn, Klaus Blaschek, Wolfgang |
| description | An arabinogalactan-protein (AGP) from pressed juice of
Echinacea purpurea herb was isolated from a high molecular weight fraction by precipitation with the β-glucosyl Yariv reagent, followed by gel-permeation chromatography. It revealed characteristic features of other AGPs: i.e., a high amount of polysaccharide (83%) with a ratio of galactose to arabinose of 1.8:1, some uronic acids (4–5%), and a low protein content (7%) with high levels of serine, alanine and hydroxyproline. The molecular weight was estimated to be 1.2×10
6 Da. Linkage and
13C NMR analyses showed that the AGP is composed of a highly branched core polysaccharide of 3-, 6-, and 3,6-linked Gal
p residues with terminal Ara
f, GlcA
p and terminal units of Ara
f-(1→5)-Ara
f-(1→. Partial acid hydrolysis resulted in loss of Ara
f residues at the periphery of the molecule. Complete loss of reactivity toward the β-glucosyl Yariv antigen was then noticed. |
| doi_str_mv | 10.1016/S0008-6215(00)00074-4 |
| format | article |
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Echinacea purpurea herb was isolated from a high molecular weight fraction by precipitation with the β-glucosyl Yariv reagent, followed by gel-permeation chromatography. It revealed characteristic features of other AGPs: i.e., a high amount of polysaccharide (83%) with a ratio of galactose to arabinose of 1.8:1, some uronic acids (4–5%), and a low protein content (7%) with high levels of serine, alanine and hydroxyproline. The molecular weight was estimated to be 1.2×10
6 Da. Linkage and
13C NMR analyses showed that the AGP is composed of a highly branched core polysaccharide of 3-, 6-, and 3,6-linked Gal
p residues with terminal Ara
f, GlcA
p and terminal units of Ara
f-(1→5)-Ara
f-(1→. Partial acid hydrolysis resulted in loss of Ara
f residues at the periphery of the molecule. Complete loss of reactivity toward the β-glucosyl Yariv antigen was then noticed.</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>DOI: 10.1016/S0008-6215(00)00074-4</identifier><identifier>PMID: 10990035</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Arabinogalactan-proteins ; Carbohydrate Conformation ; Chemical Precipitation ; Chromatography, Gel ; Echinacea - chemistry ; Echinacea purpurea ; Galactans - analysis ; Galactans - chemistry ; Galactans - isolation & purification ; Glucosides ; Glycoproteins - analysis ; Glycoproteins - chemistry ; Glycoproteins - isolation & purification ; Indicators and Reagents ; Magnetic Resonance Spectroscopy ; Molecular Weight ; Phloroglucinol - analogs & derivatives ; Plant Extracts - chemistry ; Plants, Medicinal ; Structural analysis ; β-Glucosyl Yariv reagent</subject><ispartof>Carbohydrate research, 2000-08, Vol.327 (4), p.497-504</ispartof><rights>2000 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-8ceb97162bd6423cc19f6cd38a41b1353899c1429b7ed590a9dffcbe8479f2743</citedby><cites>FETCH-LOGICAL-c361t-8ceb97162bd6423cc19f6cd38a41b1353899c1429b7ed590a9dffcbe8479f2743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10990035$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Classen, Birgit</creatorcontrib><creatorcontrib>Witthohn, Klaus</creatorcontrib><creatorcontrib>Blaschek, Wolfgang</creatorcontrib><title>Characterization of an arabinogalactan-protein isolated from pressed juice of Echinacea purpurea by precipitation with the β-glucosyl Yariv reagent</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>An arabinogalactan-protein (AGP) from pressed juice of
Echinacea purpurea herb was isolated from a high molecular weight fraction by precipitation with the β-glucosyl Yariv reagent, followed by gel-permeation chromatography. It revealed characteristic features of other AGPs: i.e., a high amount of polysaccharide (83%) with a ratio of galactose to arabinose of 1.8:1, some uronic acids (4–5%), and a low protein content (7%) with high levels of serine, alanine and hydroxyproline. The molecular weight was estimated to be 1.2×10
6 Da. Linkage and
13C NMR analyses showed that the AGP is composed of a highly branched core polysaccharide of 3-, 6-, and 3,6-linked Gal
p residues with terminal Ara
f, GlcA
p and terminal units of Ara
f-(1→5)-Ara
f-(1→. Partial acid hydrolysis resulted in loss of Ara
f residues at the periphery of the molecule. Complete loss of reactivity toward the β-glucosyl Yariv antigen was then noticed.</description><subject>Arabinogalactan-proteins</subject><subject>Carbohydrate Conformation</subject><subject>Chemical Precipitation</subject><subject>Chromatography, Gel</subject><subject>Echinacea - chemistry</subject><subject>Echinacea purpurea</subject><subject>Galactans - analysis</subject><subject>Galactans - chemistry</subject><subject>Galactans - isolation & purification</subject><subject>Glucosides</subject><subject>Glycoproteins - analysis</subject><subject>Glycoproteins - chemistry</subject><subject>Glycoproteins - isolation & purification</subject><subject>Indicators and Reagents</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Molecular Weight</subject><subject>Phloroglucinol - analogs & derivatives</subject><subject>Plant Extracts - chemistry</subject><subject>Plants, Medicinal</subject><subject>Structural analysis</subject><subject>β-Glucosyl Yariv reagent</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkUFu1DAUhi1ERYfCEUBeIViktZ3EjlcVGhVaqVIXFAlWluO8zLjK2MF2iqbn6El6EM6E01QVOyRL9m9_z7_e_xB6R8kxJZSffCOENAVntP5IyKcsRFVUL9CKNqIsKsZ_vESrZ-QQvY7xJkvCBX-FDimRkpCyXqH79VYHbRIEe6eT9Q77HmuH82Vrnd_oIT9qV4zBJ7AO2-gHnaDDffA7PAaIMYubyRqYK8_M1jptQONxCnnlQ7ufMWNHmxaD3zZtcdoC_vNQbIbJ-Lgf8E8d7C3O_AZceoMOej1EePu0H6HvX86u1-fF5dXXi_Xny8KUnKaiMdBKQTlrO16x0hgqe266stEVbWlZl42UhlZMtgK6WhItu743LTSVkD0TVXmEPiz_5u5-TRCT2tloYBi0Az9FJRirG0ZFBusFNMHHGKBXY7A7HfaKEjWPQz2OQ81ZK0LU4zjUbPD-yWBqd9D9U7Xkn4HTBYDc5q2FoKKx4Ax0NmeWVOftfyz-AvjGnlY</recordid><startdate>20000807</startdate><enddate>20000807</enddate><creator>Classen, Birgit</creator><creator>Witthohn, Klaus</creator><creator>Blaschek, Wolfgang</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000807</creationdate><title>Characterization of an arabinogalactan-protein isolated from pressed juice of Echinacea purpurea by precipitation with the β-glucosyl Yariv reagent</title><author>Classen, Birgit ; Witthohn, Klaus ; Blaschek, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-8ceb97162bd6423cc19f6cd38a41b1353899c1429b7ed590a9dffcbe8479f2743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Arabinogalactan-proteins</topic><topic>Carbohydrate Conformation</topic><topic>Chemical Precipitation</topic><topic>Chromatography, Gel</topic><topic>Echinacea - chemistry</topic><topic>Echinacea purpurea</topic><topic>Galactans - analysis</topic><topic>Galactans - chemistry</topic><topic>Galactans - isolation & purification</topic><topic>Glucosides</topic><topic>Glycoproteins - analysis</topic><topic>Glycoproteins - chemistry</topic><topic>Glycoproteins - isolation & purification</topic><topic>Indicators and Reagents</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Molecular Weight</topic><topic>Phloroglucinol - analogs & derivatives</topic><topic>Plant Extracts - chemistry</topic><topic>Plants, Medicinal</topic><topic>Structural analysis</topic><topic>β-Glucosyl Yariv reagent</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Classen, Birgit</creatorcontrib><creatorcontrib>Witthohn, Klaus</creatorcontrib><creatorcontrib>Blaschek, Wolfgang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Classen, Birgit</au><au>Witthohn, Klaus</au><au>Blaschek, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of an arabinogalactan-protein isolated from pressed juice of Echinacea purpurea by precipitation with the β-glucosyl Yariv reagent</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>2000-08-07</date><risdate>2000</risdate><volume>327</volume><issue>4</issue><spage>497</spage><epage>504</epage><pages>497-504</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><abstract>An arabinogalactan-protein (AGP) from pressed juice of
Echinacea purpurea herb was isolated from a high molecular weight fraction by precipitation with the β-glucosyl Yariv reagent, followed by gel-permeation chromatography. It revealed characteristic features of other AGPs: i.e., a high amount of polysaccharide (83%) with a ratio of galactose to arabinose of 1.8:1, some uronic acids (4–5%), and a low protein content (7%) with high levels of serine, alanine and hydroxyproline. The molecular weight was estimated to be 1.2×10
6 Da. Linkage and
13C NMR analyses showed that the AGP is composed of a highly branched core polysaccharide of 3-, 6-, and 3,6-linked Gal
p residues with terminal Ara
f, GlcA
p and terminal units of Ara
f-(1→5)-Ara
f-(1→. Partial acid hydrolysis resulted in loss of Ara
f residues at the periphery of the molecule. Complete loss of reactivity toward the β-glucosyl Yariv antigen was then noticed.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>10990035</pmid><doi>10.1016/S0008-6215(00)00074-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | Carbohydrate research, 2000-08, Vol.327 (4), p.497-504 |
| issn | 0008-6215 1873-426X |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Arabinogalactan-proteins Carbohydrate Conformation Chemical Precipitation Chromatography, Gel Echinacea - chemistry Echinacea purpurea Galactans - analysis Galactans - chemistry Galactans - isolation & purification Glucosides Glycoproteins - analysis Glycoproteins - chemistry Glycoproteins - isolation & purification Indicators and Reagents Magnetic Resonance Spectroscopy Molecular Weight Phloroglucinol - analogs & derivatives Plant Extracts - chemistry Plants, Medicinal Structural analysis β-Glucosyl Yariv reagent |
| title | Characterization of an arabinogalactan-protein isolated from pressed juice of Echinacea purpurea by precipitation with the β-glucosyl Yariv reagent |
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