Antiproliferative effects and insulin-like growth factor-I expression in Balb-C 3T3 fibroblasts after treatment with somatostatin and gonadotropin-releasing hormone analogs

The present study was aimed to compare antiproliferative effects of somatostatin (SS) and gonadotropin-releasing hormone analogs (GnRHa) on a fibroblast cell line. Proliferation index, cell count, viability of the cells and insulin-like growth factor-I (IGF-I) immunoreactivity were determined after...

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Bibliographic Details
Published in:Acta histochemica 2000, Vol.102 (3), p.353-363
Main Authors: Vural, Birol, Solakoğlu, Seyhun, Vural, Fisun, Dalçik, Cannur, Erk, Ahmet, Yücesoy, İzzet
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Cell Count
Cell Division - drug effects
Cell Survival
fibroblast
Fibroblasts - metabolism
gonadotropin releasing hormon analogs (GnRHa)
Gonadotropin-Releasing Hormone - analogs & derivatives
Gonadotropin-Releasing Hormone - pharmacology
Immunohistochemistry
Insulin-Like Growth Factor I - biosynthesis
insulin-like growth factor-I (IGF-I)
Mice
Mice, Inbred BALB C
proliferation
somatostatin
Somatostatin - analogs & derivatives
Somatostatin - pharmacology
Time Factors
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Summary:The present study was aimed to compare antiproliferative effects of somatostatin (SS) and gonadotropin-releasing hormone analogs (GnRHa) on a fibroblast cell line. Proliferation index, cell count, viability of the cells and insulin-like growth factor-I (IGF-I) immunoreactivity were determined after treatment with either SS (100 µM/ml), GnRHa (35nM/ml) or SS and GnRHa of Balb-C 3T3 mouse fibroblasts. It was found that the proliferation index, cell count, viability and IGF-I immunoreactivity were not affected by GnRHa treatment as compared with no treatment (p>0.05). Application of SS to the fibroblasts resulted in a significant reduction in proliferation index, cell count, and IGF-I immunoreactivity as compared with GnRHa treatment and no treatment, but it had no effect on cell viability. The labelling index in SS-treated cells was significantly reduced as compared with combined treatment with SS and GnRHa. In conclusion, a direct effect of GnRHa on fibroblast cells in culture could not be demonstrated. SS had direct inhibitory effects on cell proliferation possibly via inhibition of IGF-I effects without affecting cell viability.
ISSN:0065-1281
1618-0372
DOI:10.1078/S0065-1281(04)70041-0