Rapid epitope identification from complex class-II-restricted T-cell antigens

The identification of CD4 + T-cell epitopes is a laborious and tedious process. Various computer-assisted algorithms have been designed to assist in this process by predicting potential MHC-binding epitopes. Recently, the novel approach of using soluble MHC class II tetramers as a tool to identify T...

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Bibliographic Details
Published in:Trends in immunology 2001-11, Vol.22 (11), p.583-588
Main Authors: Kwok, William W., Gebe, John A., Liu, Andrew, Agar, Stacy, Ptacek, Nancy, Hammer, Juergen, Koelle, David M., Nepom, Gerald T.
Format: Article
Language:English
Subjects:
Algorithms
Alleles
Amino Acid Sequence
Epitope Mapping - methods
Epitopes, T-Lymphocyte - chemistry
Epitopes, T-Lymphocyte - immunology
Flow Cytometry
Histocompatibility Antigens Class II - immunology
Humans
MHC class II tetramers
Molecular Sequence Data
Software
T cell epitopes
T-Lymphocytes - immunology
TEPITOPE
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Summary:The identification of CD4 + T-cell epitopes is a laborious and tedious process. Various computer-assisted algorithms have been designed to assist in this process by predicting potential MHC-binding epitopes. Recently, the novel approach of using soluble MHC class II tetramers as a tool to identify T-cell epitopes has been developed successfully. We show that the T-cell epitopes identified using MHC tetramers are also predicted by a computer-assisted algorithm to bind to the same MHC molecule. We propose that epitope mapping using the MHC-tetramer-guided approach, in conjunction with predictive computer algorithms, will allow the rapid identification of T-cell epitopes from large proteins.
ISSN:1471-4906
1471-4981
DOI:10.1016/S1471-4906(01)02038-5