Growth and Anthraquinone Production of Morinda elliptica Cell Suspension Cultures in a Stirred-Tank Bioreactor

The effects of medium strategy, number of impellers, aeration mode, and mode of operation on Morinda elliptica cell suspension cultures in a stirred-tank bioreactor are described. A lower number of impellers and continuous aeration contributed toward high cell growth rate, whereas a higher number of...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2000-09, Vol.48 (9), p.4432-4438
Main Authors: Abdullah, Mohd. A, Ariff, Arbakariya B, Marziah, Mahmood, Ali, Abdul M, Lajis, Nordin H
Format: Article
Language:English
Subjects:
Anthraquinones - metabolism
Biological and medical sciences
Bioreactors
Biotechnology
Cell Culture Techniques
Establishment of new cell lines, improvement of cultural methods, mass culture
Eukaryotic cell cultures
Food additives
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Methods. Procedures. Technologies
Plant Cells
Plant cells and fungal cells
Plants - metabolism
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Summary:The effects of medium strategy, number of impellers, aeration mode, and mode of operation on Morinda elliptica cell suspension cultures in a stirred-tank bioreactor are described. A lower number of impellers and continuous aeration contributed toward high cell growth rate, whereas a higher number of impellers reduced cell growth rate, although not anthraquinone yield. The semicontinuous mode could indirectly imitate the larger scale version of production medium strategy and improved anthraquinone production even with 0.012% (v/v) antifoam addition. Production medium promoted both growth (maximum dry cell weight of 24.6 g/L) and anthraquinone formation (maximum content of 19.5 mg/g of dry cell weight), without any necessity for antifoam addition. Cultures in production medium or with higher growth rate and anthraquinone production were less acidic than cultures in growth medium or with lower growth rate and anthraquinone production. Using the best operating variables, growth of M. elliptica cells (24.6 g/L) and anthraquinone yield (0.25 g/L) were 45% and 140%, respectively, lower than those using a shake flask culture after 12 days of cultivation. Keywords: Plant cell culture; Morinda elliptica; anthraquinone; colorant; bioreactor; medium strategy
ISSN:0021-8561
1520-5118
DOI:10.1021/jf991014v