Immunization of cattle with Anaplasma marginale derived from tick cell culture

Anaplasmosis is a hemolytic disease of cattle caused by the ehrlichial tick-borne pathogen Anaplasma marginale. Killed vaccines used for control of anaplasmosis in the US used antigen harvested from infected bovine erythrocytes which was often contaminated with bovine cells and other pathogens. In t...

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Bibliographic Details
Published in:Veterinary parasitology 2001-12, Vol.102 (1), p.151-161
Main Authors: Kocan, Katherine M., Halbur, Thomas, Blouin, Edmour F., Onet, Virginia, de la Fuente, José, Garcia-Garcia, Jose C., Saliki, Jeremiah T.
Format: Article
Language:English
Subjects:
Anaplasma - immunology
Anaplasma - pathogenicity
Anaplasma marginale
Anaplasmosis - immunology
Anaplasmosis - prevention & control
Animals
Antibodies, Bacterial - blood
Bacterial Outer Membrane Proteins - immunology
Bacterial Vaccines
Cattle
Cattle Diseases - immunology
Cattle Diseases - microbiology
Cattle Diseases - prevention & control
Cell Line
Ehrlichiae
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Immunization
Injections, Subcutaneous - veterinary
Ixodes - embryology
Major surface protein
Tick cell culture
Vaccination - methods
Vaccination - veterinary
Vaccine
Vaccines, Inactivated
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Summary:Anaplasmosis is a hemolytic disease of cattle caused by the ehrlichial tick-borne pathogen Anaplasma marginale. Killed vaccines used for control of anaplasmosis in the US used antigen harvested from infected bovine erythrocytes which was often contaminated with bovine cells and other pathogens. In this study, we performed an initial cattle trial to test A. marginale harvested from tick cell culture as an immunogen for cattle. Eleven yearling Holstein cattle were immunized with the cell culture-derived A. marginale and 11 cattle were non-immunized contact controls. Each vaccine dose contained approximately 2×10 10 A. marginale in an oil-based adjuvant. Two immunizations were administered subcutaneously 4 weeks apart and the cattle were challenge-exposed 10 weeks after the second immunization with A. marginale infected blood. Maximum antibody levels as determined by an A. marginale specific competitive ELISA were observed 2 weeks after the last immunization. Antibody responses against major surface proteins (MSPs) 1a and 1β1 were also characterized and immunized cattle demonstrated a preferential recognition for MSP1β1. Cattle immunized with the cell culture-derived A. marginale had a significantly lower percent reduction in the packed cell volume ( P
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(01)00519-2