Characterization of protein C receptor expression in monocytes

Many sequelae associated with endotoxaemic‐induced shock result from excessive production of the cytokine mediators, tumour necrosis factor alpha (TNF‐α), interleukin 1 (IL‐1) and IL‐6 from lipopolysaccharide (LPS)‐activated monocytes. Protein C (PC)/activated protein C (APC) has potent cytokine‐mod...

Full description

Saved in:
Bibliographic Details
Published in:British journal of haematology 2001-11, Vol.115 (2), p.408-414
Main Authors: Galligan, Leeona, Livingstone, Wendy, Volkov, Yuri, Hokamp, Karsten, Murphy, Ciaran, Lawler, Mark, Fukudome, Kenji, Smith, Owen
Format: Article
Language:English
Subjects:
activation
Biological and medical sciences
Blood Coagulation Factors
Databases, Genetic
DNA, Complementary - genetics
Fluorescent Antibody Technique, Indirect
General aspects
Hematology
Humans
Infection pathogenesis
Infectious diseases
Medical sciences
monocyte
Monocytes - metabolism
protein C
receptor
Receptors, Cell Surface - blood
Receptors, Cell Surface - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
sepsis
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Many sequelae associated with endotoxaemic‐induced shock result from excessive production of the cytokine mediators, tumour necrosis factor alpha (TNF‐α), interleukin 1 (IL‐1) and IL‐6 from lipopolysaccharide (LPS)‐activated monocytes. Protein C (PC)/activated protein C (APC) has potent cytokine‐modifying properties and is protective in animal models and human clinical trials of sepsis. The precise mechanism by which this anti‐inflammatory response is achieved remains unknown; however, the recently described endothelial protein C receptor (EPCR) appears to be essential for this function. The pivotal role that monocytes play in the pathophysiology of septic shock led us to investigate the possible expression of a protein C receptor on the monocyte membrane. We used similarity algorithms to screen human sequence databases for paralogues of the EPCR but found none. However, using reverse transcription–polymerase chain reaction (RT–PCR), we detected an mRNA transcribed in primary human monocytes and THP1 cells that was identical to human EPCR mRNA. We also used immunocytochemical analysis to demonstrate the expression of a protein C receptor on the surface of monocytes encoded by the same gene as EPCR. These results confirm a new member of the protein C pathway involving primary monocytes. Further characterization will be necessary to compare and contrast its biological properties with those of EPCR.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.2001.03187.x