Coupling High-Pressure MALDI with Ion Mobility/Orthogonal Time-of-Flight Mass Spectrometry

A new ion mobility/time-of-flight mass spectrometer employing a high-pressure MALDI source has been designed and tested. The prototype instrument operates at a source/drift cell pressure of 1−10 Torr helium, resulting in a mobility resolution of ∼25. A small time-of-flight mass spectrometer (20 cm)...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2000-09, Vol.72 (17), p.3965-3971
Main Authors: Gillig, Kent J, Ruotolo, Brandon, Stone, Earle G, Russell, David H, Fuhrer, Katrin, Gonin, Marc, Schultz, Albert J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Fundamental and applied biological sciences. Psychology
Ions
Mass Spectrometry - instrumentation
Molecular Sequence Data
Peptides - chemistry
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:A new ion mobility/time-of-flight mass spectrometer employing a high-pressure MALDI source has been designed and tested. The prototype instrument operates at a source/drift cell pressure of 1−10 Torr helium, resulting in a mobility resolution of ∼25. A small time-of-flight mass spectrometer (20 cm) with a mass resolution of up to 200 has been attached to the drift cell to identify (in terms of mass-to-charge ratio) the separated ions. A simple tripeptide mixture has been separated in the drift tube and mass identified as singly protonated species. The ability to separate peptide mixtures, e.g., tryptic digest of a protein, is illustrated and compared to results obtained on a high-vacuum time-of-flight instrument.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0005619