Histochemical differentiation of Dirofilaria immitis, Dirofilaria repens and Acanthocheilonema dracunculoides microfilariae by staining with a commercial kit, Leucognost-SP

The diagnosis of canine heartworm infection is based upon the presence of circulating Dirofilaria immitis microfilariae or on techniques for the detection of serum antibodies or antigens. In the first of these, discrimination between D. immitis, D. repens and Acanthocheilonema dracunculoides microfi...

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Bibliographic Details
Published in:Veterinary parasitology 2001-12, Vol.102 (1), p.173-175
Main Authors: Peribáñez, Miguel A., Lucientes, Javier, Arce, Sergio, Morales, Mariano, Castillo, Juan A., Gracia, Marı́a J.
Format: Article
Language:English
Subjects:
Acanthocheilonema sp
Acid phosphatase
Acid Phosphatase - analysis
Animals
Diagnosis, Differential
Dipetalonema - classification
Dipetalonema - enzymology
Dipetalonema Infections - diagnosis
Dipetalonema Infections - veterinary
Dirofilaria - classification
Dirofilaria - enzymology
Dirofilaria immitis - classification
Dirofilaria immitis - enzymology
Dirofilaria sp
Dirofilariasis - diagnosis
Dog Diseases - diagnosis
Dog Diseases - parasitology
Dogs
Heartworm disease
Histocytochemistry - methods
Histocytochemistry - veterinary
Leucognost-SP
Microfilariae - classification
Microfilariae - enzymology
Reagent Kits, Diagnostic - veterinary
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Summary:The diagnosis of canine heartworm infection is based upon the presence of circulating Dirofilaria immitis microfilariae or on techniques for the detection of serum antibodies or antigens. In the first of these, discrimination between D. immitis, D. repens and Acanthocheilonema dracunculoides microfilariae is based upon the acid phosphatase histochemical stain. In this paper, we propose an alternative technique for histochemical staining using a commercial kit test of naphthol-AS-OL (Leucognost-SP ®). This offers the advantages of speed and simplicity as compared to the standard Barka procedure.
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(01)00516-7