Application of capillary electrophoresis in clinical chemistry: the clinical value of urinary modified nucleosides

Urinary modified nucleosides were determined by capillary electrophoresis using a 300 m M SDS–25 m M sodium tetraborate–50 m M sodium dihydrogenphosphate buffer. The nucleosides were extracted from urine by phenylboronate affinity gel chromatography. In cancer patients the levels of the modified nuc...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2000-08, Vol.745 (1), p.189-196
Main Authors: Liebich, H.M, Lehmann, R, Xu, G, Wahl, H.G, Häring, H.-U
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Case-Control Studies
Chemistry, Clinical
Chromatography, Affinity
Chromatography, Gel
Electrophoresis, Capillary - methods
Female
Fundamental and applied biological sciences. Psychology
Humans
Male
Neoplasms - urine
Nucleic acids
Nucleic bases, nucleotides
Nucleosides
Nucleosides - isolation & purification
Nucleosides - urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Urinary modified nucleosides were determined by capillary electrophoresis using a 300 m M SDS–25 m M sodium tetraborate–50 m M sodium dihydrogenphosphate buffer. The nucleosides were extracted from urine by phenylboronate affinity gel chromatography. In cancer patients the levels of the modified nucleosides are generally elevated. By an artificial neural network method breast cancer patients were differentiated from normal individuals, which indicates that the modified nucleosides could be of clinical value as tumor markers.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(00)00263-2