Mass spectrometric methods for the characterisation and differentiation of isomeric O-diglycosyl flavonoids

Tandem mass spectrometric methods have been evaluated for the characterisation of the type and the differentiation of the interglycosidic linkage of isomeric flavonoid O‐diglycosides. Based on the occurrence of internal monosaccharide residue loss and the relative abundances of Y‐type ions formed by...

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Bibliographic Details
Published in:Phytochemical analysis 2001-05, Vol.12 (3), p.159-165
Main Authors: Ma, Yu-Liang, Cuyckens, Filip, Heuvel, Hilde Van den, Claeys, Magda
Format: Article
Language:English
Subjects:
Carbohydrate Conformation
Carbohydrate Sequence
electrospray ionization
Fast atom bombardment
flavonoid glycosides
Flavonoids - chemistry
Glycosides - chemistry
internal monosaccharide residue loss
Isomerism
Mass Spectrometry - methods
metastable ion decomposition
tandem mass spectrometry
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Summary:Tandem mass spectrometric methods have been evaluated for the characterisation of the type and the differentiation of the interglycosidic linkage of isomeric flavonoid O‐diglycosides. Based on the occurrence of internal monosaccharide residue loss and the relative abundances of Y‐type ions formed by fragmentation at glycosidic bonds, four pairs of isomeric flavonoid O‐diglycosides can be unambiguously differentiated. The different techniques used, i.e. linked scanning at constant B/E without collisional activation and low‐energy collision‐induced dissociation using methane or helium as collision gas, have been shown to be useful for distinguishing the two most common (1, 2‐ and 1, 6‐) interglycosidic linkages, e.g. flavonoid O‐neohesperidosides and O‐rutinosides. Copyright © 2001 John Wiley & Sons, Ltd.
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.573