Flow cytometric analysis of defensins in blood and marrow neutrophils

: Polymorphonuclear neutrophils (PMN) are vital in host defense against microbial infections. This study provides a flow cytometric method for the quantitative analysis of microbicidal peptides (defensins) in cells of PMN lineage. Rabbit neutrophil peptides, NP‐2 and NP‐5, were measured in all PMN a...

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Bibliographic Details
Published in:European journal of haematology 2000-02, Vol.64 (2), p.114-120
Main Authors: Klut, Maria E., Whalen, Beth A., Hogg, James C.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase
Animals
Biological and medical sciences
blood
Bone Marrow Cells - chemistry
Cytoplasmic Granules - chemistry
Defensins
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Immunoenzyme Techniques
Immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
Leukocyte Count
marrow
Medical sciences
Miscellaneous. Technology
Myeloid cells: ontogeny, maturation, markers, receptors
Neutrophils - chemistry
Neutrophils - ultrastructure
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
polymorphonuclear neutrophils
Polynuclears
Proteins - analysis
Rabbits
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Summary:: Polymorphonuclear neutrophils (PMN) are vital in host defense against microbial infections. This study provides a flow cytometric method for the quantitative analysis of microbicidal peptides (defensins) in cells of PMN lineage. Rabbit neutrophil peptides, NP‐2 and NP‐5, were measured in all PMN and in subpopulations of PMN expressing l‐selectin. PMN lineage counts were made on Wright's‐stained blood smears and marrow cytospins. Immunoreactivity for NP‐2, and NP‐5 was detected by using the alkaline phosphatase anti‐alkaline phosphatase technique. The results show that marrow PMN express higher levels of NP‐2 and NP‐5 than blood PMN, p
ISSN:0902-4441
1600-0609
DOI:10.1034/j.1600-0609.2000.90069.x