Detection of Gov system antibodies by MAIPA reveals an immunogenicity similar to the HPA‐5 alloantigens

The glycosylphosphatidylinositol‐linked platelet protein CD109 carries the biallelic alloantigen system Gov. There is limited information on the incidence of Gov alloantibodies in neonatal alloimmune thrombocytopenia (NAITP), post‐transfusion purpura (PTP) and platelet refractoriness. We adapted the...

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Bibliographic Details
Published in:British journal of haematology 2000-09, Vol.110 (3), p.735-742
Main Authors: Berry, J. E., Murphy, C. M., Smith, G. A., Ranasinghe, E., Finberg, R., Walton, J., Brown, J., Navarrete, C., Metcalfe, P., Ouwehand, W. H.
Format: Article
Language:English
Subjects:
Alleles
Antibodies, Monoclonal
Antigens, Human Platelet - immunology
Biological and medical sciences
Cryopreservation
Genotype
Gov system
Hematologic and hematopoietic diseases
Hematology
Histocompatibility Antigens Class II - analysis
Humans
immunogenicity
Isoantibodies - analysis
Medical sciences
monoclonal antibody‐specific immobilization of platelet antigens (MAIPA)
Platelet diseases and coagulopathies
platelets
Thrombocytopenia - immunology
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Summary:The glycosylphosphatidylinositol‐linked platelet protein CD109 carries the biallelic alloantigen system Gov. There is limited information on the incidence of Gov alloantibodies in neonatal alloimmune thrombocytopenia (NAITP), post‐transfusion purpura (PTP) and platelet refractoriness. We adapted the monoclonal antibody‐specific immobilization of platelet antigens (MAIPA) assay to the detection of Gov antibodies and determined their incidence in 605 archived samples (112 with HPA antibodies) referred for the aforementioned conditions. Here, we show that CD109 expression was reduced upon platelet storage in saline or by cryopreservation, but was stable when stored as whole blood or therapeutic platelet concentrate. Fourteen of the 605 samples contained Gov alloantibodies (anti‐Gova, n = 10; anti‐Govb, n = 4), with the majority in platelet refractoriness (n = 9) and, of the remaining five, four in NAITP and one in PTP. In seven cases, no other HPA antibodies were detected, three being NAITP cases. The incidence of Gov antibodies was significantly lower than HPA‐1 system antibodies (n = 87), but equalled the number of HPA‐5 system antibodies (n = 14) and outnumbered HPA‐2 and ‐3 system antibodies (10 altogether).
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.2000.02170.x