Dietary fish oil reduces intercellular adhesion molecule 1 and scavenger receptor expression on murine macrophages

• Published in: Atherosclerosis 2000-09, Vol.152 (1), p.43-50
• Main Authors: Miles, Elizabeth A., Wallace, Fiona A., Calder, Philip C.
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Atherosclerosis; Atherosclerosis (general aspects, experimental research); Base Sequence; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Cells, Cultured; Chromatography, Gas; Coconut Oil; Corn Oil - pharmacology; Diet, Fat-Restricted; Fish oil; Fish Oils - pharmacology; Flow Cytometry; Intercellular adhesion molecule 1; Intercellular Adhesion Molecule-1 - analysis; Intercellular Adhesion Molecule-1 - drug effects; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - metabolism; Male; Medical sciences; Membrane Proteins; Mice; Mice, Inbred C57BL; Models, Animal; Molecular Sequence Data; Murine peritoneal macrophage; Plant Oils - pharmacology; Polymerase Chain Reaction; Probability; Receptors, Immunologic - analysis; Receptors, Immunologic - drug effects; Receptors, Lipoprotein; Receptors, Scavenger; Reference Values; RNA, Messenger - analysis; Safflower Oil - pharmacology; Scavenger receptor; Scavenger Receptors, Class B
• ISSN: 0021-9150; 1879-1484
• DOI: 10.1016/S0021-9150(99)00446-3

During atherogenesis, a pathological accumulation of lipids occurs within aortic intimal macrophages through uptake of oxidised low-density lipoprotein (LDL) via scavenger receptors. Here we investigate whether some of the anti-atherosclerotic effects ascribed to dietary fish oil are mediated through effects on macrophage intercellular adhesion molecule 1 (ICAM-1) and scavenger receptor expression. Mice were fed on a low fat diet (containing 25 g/kg corn oil) or on high fat diets containing 200 g/kg coconut oil, safflower oil or fish oil. Thioglycollate-elicited peritoneal macrophages were analysed for fatty acid composition by gas chromatography. Macrophage scavenger receptor A (MSR-A) type I+type II and ICAM-1 expression were measured by flow cytometry and the levels of mRNA coding for MSR-A type I, MSR-A type II and ICAM-1 were measured by reverse-transcription polymerase chain reaction. Feeding mice diets enriched with different fats resulted in significant changes in the fatty acid profile of macrophages, which reflected the fatty acid compositions of the diets. Macrophages from the fish oil fed mice had the lowest expression of ICAM-1 and MSR-A at the level of both mRNA and cell surface expression. The reduced expression of ICAM-1 and MSR-A on macrophages from mice fed on a fish oil-rich diet supports our hypothesis that part of the protective effect of fish oil against atherosclerosis might be due to an altered macrophage phenotype and function ameliorating macrophage-induced plaque formation.