Chemosensitivity in childhood brain tumours in vitro: evidence of differential sensitivity to lomustine (CCNU) and vincristine

The aim of this study was to examine the range of sensitivity of a panel of short-term cultures derived from different types of malignant childhood brain tumours including medulloblastoma, ependymoma and glioblastoma multiforme to three cytotoxic drugs, lomustine (CCNU), vincristine (VCR) and procar...

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Bibliographic Details
Published in:European journal of cancer (1990) 2000-10, Vol.36 (15), p.1955-1964
Main Authors: Lewandowicz, G.M., Harding, B., Harkness, W., Hayward, R., Thomas, D.G.T., Darling, J.L.
Format: Article
Language:English
Subjects:
Adult
Antineoplastic agents
Antineoplastic Agents - therapeutic use
Astrocytoma
Astrocytoma - drug therapy
Biological and medical sciences
Brain Neoplasms - drug therapy
Brain tumour
Cell culture
Central neuroblastoma
Chemosensitivity
Chemotherapy
Child
Dimethylthiazolyl-2,5-diphenyl tetrazolium bromide (MTT) assay
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Ependymoma
Ependymoma - drug therapy
Female
Glioblastoma - drug therapy
Humans
Lomustine - therapeutic use
Male
Medical sciences
Medulloblastoma
Medulloblastoma - drug therapy
Neurology
Pharmacology. Drug treatments
Procarbazine - therapeutic use
Tumor Cells, Cultured - drug effects
Tumors of the nervous system. Phacomatoses
Vincristine - therapeutic use
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Summary:The aim of this study was to examine the range of sensitivity of a panel of short-term cultures derived from different types of malignant childhood brain tumours including medulloblastoma, ependymoma and glioblastoma multiforme to three cytotoxic drugs, lomustine (CCNU), vincristine (VCR) and procarbazine (PCB). Sensitivity was assessed using a modification of the dimethylthiazolyl-2,5-diphenyl tetrazolium bromide (MTT) assay. Short-term cell lines derived from ependymomas were considerably more resistant to VCR than other types of childhood brain tumours, while cultures derived from supratentorial primitive neuroectodermal tumour (PNET) displayed marked sensitivity to both lomustine and VCR. Cultures from ependymomas, medulloblastoma and astrocytic gliomas had similar sensitivity to lomustine and PCB as cultures derived from adult malignant astrocytoma.
ISSN:0959-8049
1879-0852
DOI:10.1016/S0959-8049(00)00245-8