Hepatitis B virus X protein in the proteasome of mammalian cells: defining the targeting domain

The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HB...

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Bibliographic Details
Published in:Molecular biology reports 2001-03, Vol.28 (1), p.31-34
Main Authors: Chen, W N, Oon, C J, Goo, K S
Format: Article
Language:English
Subjects:
Animals
AX protein
Carcinoma, Hepatocellular
Cell Cycle
Cysteine Endopeptidases - metabolism
Green Fluorescent Proteins
Hepatitis B virus
Humans
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Mammals
Multienzyme Complexes - metabolism
Proteasome Endopeptidase Complex
proteasomes
Proteins
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Sequence Deletion
Trans-Activators - genetics
Trans-Activators - metabolism
Tumor Cells, Cultured
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Summary:The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HBX is available. We report here the localization of HBX in the proteasome complexes using green fluorescent protein tag. A new proteasome-targeting domain has also been defined in HBX by deletion study. Furthermore, a functional role of HBX in the cellular processes mediated by the proteasome complexes has been suggested by its cell cycle-independent localization in the proteasome. Further analysis of the functional role of HBX in the proteasome complexes should provide more information on the underlying mechanism of HBX ativities.
ISSN:0301-4851
1573-4978
DOI:10.1023/A:1011997704112