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Hepatitis B virus X protein in the proteasome of mammalian cells: defining the targeting domain
The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HB...
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| Published in: | Molecular biology reports 2001-03, Vol.28 (1), p.31-34 |
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| creator | Chen, W N Oon, C J Goo, K S |
| description | The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HBX is available. We report here the localization of HBX in the proteasome complexes using green fluorescent protein tag. A new proteasome-targeting domain has also been defined in HBX by deletion study. Furthermore, a functional role of HBX in the cellular processes mediated by the proteasome complexes has been suggested by its cell cycle-independent localization in the proteasome. Further analysis of the functional role of HBX in the proteasome complexes should provide more information on the underlying mechanism of HBX ativities. |
| doi_str_mv | 10.1023/A:1011997704112 |
| format | article |
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The diversity of its activities may be mediated through its interaction with cellular organelles. However no clearly defined subcellular localization of HBX is available. We report here the localization of HBX in the proteasome complexes using green fluorescent protein tag. A new proteasome-targeting domain has also been defined in HBX by deletion study. Furthermore, a functional role of HBX in the cellular processes mediated by the proteasome complexes has been suggested by its cell cycle-independent localization in the proteasome. Further analysis of the functional role of HBX in the proteasome complexes should provide more information on the underlying mechanism of HBX ativities.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1023/A:1011997704112</identifier><identifier>PMID: 11710562</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Animals ; AX protein ; Carcinoma, Hepatocellular ; Cell Cycle ; Cysteine Endopeptidases - metabolism ; Green Fluorescent Proteins ; Hepatitis B virus ; Humans ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; Mammals ; Multienzyme Complexes - metabolism ; Proteasome Endopeptidase Complex ; proteasomes ; Proteins ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Sequence Deletion ; Trans-Activators - genetics ; Trans-Activators - metabolism ; Tumor Cells, Cultured</subject><ispartof>Molecular biology reports, 2001-03, Vol.28 (1), p.31-34</ispartof><rights>Kluwer Academic Publishers 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-ad67534b9e20e2b5efa91e22fde8311d09f27c168b077e30fb3939eca6f3491f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11710562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, W N</creatorcontrib><creatorcontrib>Oon, C J</creatorcontrib><creatorcontrib>Goo, K S</creatorcontrib><title>Hepatitis B virus X protein in the proteasome of mammalian cells: defining the targeting domain</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><description>The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. 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Oon, C J ; Goo, K S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-ad67534b9e20e2b5efa91e22fde8311d09f27c168b077e30fb3939eca6f3491f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>AX protein</topic><topic>Carcinoma, Hepatocellular</topic><topic>Cell Cycle</topic><topic>Cysteine Endopeptidases - metabolism</topic><topic>Green Fluorescent Proteins</topic><topic>Hepatitis B virus</topic><topic>Humans</topic><topic>Luminescent Proteins - genetics</topic><topic>Luminescent Proteins - metabolism</topic><topic>Mammals</topic><topic>Multienzyme Complexes - metabolism</topic><topic>Proteasome Endopeptidase Complex</topic><topic>proteasomes</topic><topic>Proteins</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sequence Deletion</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, W N</creatorcontrib><creatorcontrib>Oon, C J</creatorcontrib><creatorcontrib>Goo, K S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, W N</au><au>Oon, C J</au><au>Goo, K S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hepatitis B virus X protein in the proteasome of mammalian cells: defining the targeting domain</atitle><jtitle>Molecular biology reports</jtitle><addtitle>Mol Biol Rep</addtitle><date>2001-03-01</date><risdate>2001</risdate><volume>28</volume><issue>1</issue><spage>31</spage><epage>34</epage><pages>31-34</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>The hepatitis B virus X (HBX) protein has been implicated in both hepatitis B virus-related pathogenesis and also in diverse cellular processes. 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| subjects | Animals AX protein Carcinoma, Hepatocellular Cell Cycle Cysteine Endopeptidases - metabolism Green Fluorescent Proteins Hepatitis B virus Humans Luminescent Proteins - genetics Luminescent Proteins - metabolism Mammals Multienzyme Complexes - metabolism Proteasome Endopeptidase Complex proteasomes Proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Deletion Trans-Activators - genetics Trans-Activators - metabolism Tumor Cells, Cultured |
| title | Hepatitis B virus X protein in the proteasome of mammalian cells: defining the targeting domain |
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