Genetic relatedness of hepatitis A virus isolates during a community-wide outbreak

In 1993–94, a community‐wide outbreak of hepatitis A occurred in Stanislaus County, California. Stool specimens collected from a sample of 33 case patients were used to evaluate the duration of hepatitis A virus (HAV) excretion and the genetic relatedness of HAV isolates. Twenty‐four percent of the...

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Bibliographic Details
Published in:Journal of medical virology 2000-10, Vol.62 (2), p.144-150
Main Authors: Robertson, Betty H., Averhoff, Francisco, Cromeans, Theresa L., Han, Xiao-hua, Khoprasert, Boontham, Nainan, Omana V., Rosenberg, Jon, Paikoff, Lawrence, DeBess, Emilio, Shapiro, Craig N., Margolis, Harold S.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Antigens, Viral - analysis
Biological and medical sciences
Child
Child, Preschool
Disease Outbreaks
Epidemiology
Feces - virology
Female
Fundamental and applied biological sciences. Psychology
Genetic Variation
Hepatitis A - epidemiology
Hepatitis A - virology
Hepatitis A virus
Hepatovirus - genetics
Hepatovirus - isolation & purification
Human viral diseases
Humans
Infant
Infant, Newborn
Infectious diseases
Male
Medical sciences
Microbiology
Molecular Epidemiology
nucleic acid sequence
PCR
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - analysis
Sensitivity and Specificity
USA, California, Stanislaus County
Viral diseases
Viral hepatitis
Virology
virus detection
virus excretion
Virus Shedding
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Summary:In 1993–94, a community‐wide outbreak of hepatitis A occurred in Stanislaus County, California. Stool specimens collected from a sample of 33 case patients were used to evaluate the duration of hepatitis A virus (HAV) excretion and the genetic relatedness of HAV isolates. Twenty‐four percent of the patients had a stool sample positive for HAV antigen by enzyme immunoassay, whereas 91% had at least one stool positive for HAV RNA by RT‐PCR amplification. Children were found to excrete low levels of HAV RNA for up to 10 weeks after the onset of symptoms. Analysis of the HAV VP1 amino terminus and VP1/P2A regions showed that a limited number of HAV isolates circulated during the epidemic and the majority of the cases were infected with the same strain. J. Med. Virol. 62:144–150, 2000. © 2000 Wiley‐Liss, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/1096-9071(200010)62:2<144::AID-JMV4>3.0.CO;2-I