Identification of four highly conserved regions in DNA-PKcs

The gene for the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is responsible for severe combined immune deficiency (SCID) and its products associate with Ku70/Ku86 autoantigens, c-Abl, and other factors to exert its roles. Investigations to date suggest that DNA-PKcs comprises severa...

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Bibliographic Details
Published in:Immunogenetics (New York) 2000-09, Vol.51 (11), p.965-973
Main Authors: Fujimori, A, Araki, R, Fukumura, R, Ohhata, T, Takahashi, H, Kawahara, A, Tatsumi, K, Abe, M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Catalytic Domain
Cell Cycle Proteins - genetics
Cell Line
Chickens - genetics
Cloning, Molecular
Conserved Sequence
DNA, Complementary
DNA-Activated Protein Kinase
DNA-Binding Proteins
DNA-dependent protein kinase
DNA-PKc gene
Humans
Ku70 antigen
Ku70 autoantigen
Ku80 antigen
Ku86 autoantigen
MCM4 gene
Mice
Minichromosome Maintenance Complex Component 4
Molecular Sequence Data
Nuclear Proteins
Protein-Serine-Threonine Kinases - genetics
Sequence Homology, Amino Acid
Xenopus laevis
Xenopus laevis - genetics
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Summary:The gene for the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is responsible for severe combined immune deficiency (SCID) and its products associate with Ku70/Ku86 autoantigens, c-Abl, and other factors to exert its roles. Investigations to date suggest that DNA-PKcs comprises several regions which specifically interact with these known and other as yet unidentified factors. Nevertheless, the relationship between the structure and function of the DNA-PKcs molecule is poorly understood. Here we report cloning of the entire DNA-PKcs cDNA from chicken and Xenopus laevis. Comparative study of the DNA-PKcs polypeptides from four different vertebrates revealed at least three novel conserved regions in addition to the carboxyl-terminal region containing the phosphatidylinositol-3 kinase domain. We also demonstrated that the four vertebrates share the common genomic organization between a licensing factor, MCM4, and DNA-PKcs, both of which locate in a head-to-head manner within a few kilobase intervals. These data provide useful clues for the further genetic study of this huge multifunctional enzyme.
ISSN:0093-7711
1432-1211
DOI:10.1007/s002510000227