Effect of human endothelial cells on Human Bone Marrow Stromal Cell phenotype: Role of VEGF?

• Published in: Journal of cellular biochemistry 2000-12, Vol.79 (4), p.672-685
• Main Authors: Villars, F., Bordenave, L., Bareille, R., Amédée, J.
• Format: Article
• Language: English
• Subjects: Alkaline Phosphatase - metabolism; Base Sequence; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - physiology; Cell Communication - physiology; Cell Differentiation; Cell Division; co-culture; Coculture Techniques; Culture Media, Conditioned; DNA Primers - genetics; endothelial; Endothelial Growth Factors - genetics; Endothelial Growth Factors - pharmacology; Endothelial Growth Factors - physiology; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; Extracellular Matrix - physiology; Gene Expression; Humans; Lymphokines - genetics; Lymphokines - pharmacology; Lymphokines - physiology; Neovascularization, Physiologic - drug effects; osteoblast; Osteocalcin - biosynthesis; Receptor Protein-Tyrosine Kinases - genetics; Receptors, Growth Factor - genetics; Receptors, Vascular Endothelial Growth Factor; RNA, Messenger - genetics; RNA, Messenger - metabolism; vascular endothelial growth factor; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/1097-4644(20001215)79:4<672::AID-JCB150>3.0.CO;2-2

Angiogenesis is a tightly regulated process involved in growth, repair, and bone remodeling. Several studies have shown that there is a reciprocal regulation and functional relationship between endothelial cells and osteoblast‐like cells during osteogenesis, where systemic hormones and paracrine growth factors play an active role. Angiogenesis is induced by a variety of growth factors; among them vascular endothelial growth factor (VEGF) may be an important mediator for the angiogenic process involved in bone physiology. We studied the VEGF effect on osteoblast progenitor cells (Human Bone Marrow Stromal Cells: HBMSE) cultured alone or associated with endothelial cells (Human Umbilical Vein Endothelial Cells: HUVEC) in different co‐culture models (co‐culture with or without direct contact, conditioned medium), to determine the influence of VEGF on these cells and on their relationship. In agreement with other studies, we show that HBMSC express and synthesize VEGF, HUVEC conditioned medium has a proliferative effect on them, and early osteoblastic marker (Alkaline phosphatase activity) levels increase when these cells are co‐cultured with HUVEC only in direct contact. However, unlike previous studies, we did not find that VEGF increased these processes. These results suggest that the intercommunication between endothelial cells and osteoblastic‐like cells requires not only diffusible factors, but also involving cell membrane proteins. J. Cell. Biochem. 79:672–685, 2000. © 2000 Wiley‐Liss, Inc.