A simplified objective method for quantification of peste des petits ruminants virus or neutralizing antibody

• Published in: Journal of virological methods 2000-09, Vol.89 (1), p.89-95
• Main Authors: Dhinakar Raj, G, Nachimuthu, K, Mahalinga Nainar, A
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Viral - blood; Biological and medical sciences; Cercopithecus aethiops; Chickens; Cytopathic effect; Cytopathogenic Effect, Viral; Fundamental and applied biological sciences. Psychology; Haemagglutination; Haemagglutination inhibition; Hemagglutination Tests - methods; Microbiology; Neutralization Tests; Peste-des-petits-ruminants virus; Peste-des-petits-ruminants virus - growth & development; Peste-des-petits-ruminants virus - immunology; Peste-des-petits-ruminants virus - isolation & purification; Sensitivity and Specificity; Serum neutralization; Techniques used in virology; Vero Cells; Virology; Virus Replication
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(00)00206-8

A simplified and standardized assay based on haemagglutination of infected culture supernatants was developed to detect peste des petits ruminants (PPR) virus growth in vero cells and to quantify PPR neutralizing antibody. Virus titres estimated by visual reading of cytopathic effects as a criterion were compared with those estimated by haemagglutination of infected supernatants and no statistically significant differences were seen between them. During quantification of PPR antibodies, the titres based on haemagglutination of supernatants on day 5 post-infection had a high sensitivity, specificity and agreement in qualititative comparison with those determined by visual examination of cytopathic effects. In quantitative comparison, the correlation coefficient between serum neutralization titres estimated by visual examination of cytopathic effects or haemagglutination of supernatants was 0.96, when haemagglutination was done on day 5 post-infection. The virus and serum neutralization titres can thus be estimated objectively using the haemagglutination of supernatants as criterion to measure endpoints. The haemagglutination–inhibition titres also correlated well with serum neutralization titres with a coefficient of 0.78. Thus the haemagglutination–inhibition test appears to be a suitable alternative to the serum neutralization test for quantification of PPR neutralizing antibody.