Intracellular trafficking and transgene expression of viral and non-viral gene vectors

The objective of this review is to summarize the critical steps in intracellular trafficking and the principal factors involved in transgene expression by a comparison of viral and non-viral gene vectors. Intracellular trafficking of viral and non-viral gene vectors are reviewed kinetically as well...

Full description

Saved in:
Bibliographic Details
Published in:Advanced drug delivery reviews 2001-11, Vol.52 (3), p.153-164
Main Authors: Kamiya, H, Tsuchiya, H, Yamazaki, J, Harashima, H
Format: Article
Language:English
Subjects:
Animals
Biological Transport - drug effects
Biological Transport - genetics
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Drug Delivery Systems - methods
Endosomal escape
Gene Expression Regulation, Viral
Genetic Vectors - biosynthesis
Genetic Vectors - metabolism
Humans
Intracellular Fluid - metabolism
Intracellular trafficking
Nuclear transport
Transcription
Transgenes
Translation
Viral and non-viral vectors
Viruses - genetics
Viruses - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of this review is to summarize the critical steps in intracellular trafficking and the principal factors involved in transgene expression by a comparison of viral and non-viral gene vectors. Intracellular trafficking of viral and non-viral gene vectors are reviewed kinetically as well as from the mechanistic point of view. The peptide-dependent specific mechanism of endosomal escape by viral vectors is compared with the non-specific mechanism of non-viral vectors. Regarding the nuclear transport of DNA, a number of recently developed strategies in non-viral vectors such as the application of nuclear localization signals or cell specific transcription factors are summarized in comparison with viral nuclear gene delivery. The molecular mechanisms of transcription and the translation of delivered genes to nucleus are also summarized in view of drug delivery systems. This information is intended to serve as a basis for developing a new gene delivery system for both viral and non-viral gene vectors. Optimizing the gene delivery system by integrating this intracellular trafficking as well as transgene expression will be required in order to develop an efficient and an safe gene delivery system.
ISSN:0169-409X
1872-8294
DOI:10.1016/S0169-409X(01)00216-2