Measurement of the layered compressive properties of trypsin-treated articular cartilage : an ultrasound investigation

An ultrasound-compression system has been developed for the study of the layered biomechanical properties of articular cartilage. Cartilage specimens harvested from the bovine patella groove, with and without trypsin digestion, were tested using this system. It was noted that a large ultrasound refl...

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Bibliographic Details
Published in:Medical & biological engineering & computing 2001-09, Vol.39 (5), p.534-541
Main Authors: ZHENG, Y. P, DING, C. X, BAI, J, MAK, A. F. T, QIN, L
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biomechanics
Cartilage, Articular - diagnostic imaging
Cartilage, Articular - drug effects
Cartilage, Articular - physiopathology
Cattle
Compression testing
Diseases of the osteoarticular system
Elasticity - drug effects
In Vitro Techniques
Investigative techniques, diagnostic techniques (general aspects)
Joints
Medical sciences
Osteoarthritis
Patella
Stress, Mechanical
Trypsin - pharmacology
Ultrasonic investigative techniques
Ultrasonic measurement
Ultrasonography
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Summary:An ultrasound-compression system has been developed for the study of the layered biomechanical properties of articular cartilage. Cartilage specimens harvested from the bovine patella groove, with and without trypsin digestion, were tested using this system. It was noted that a large ultrasound reflection can be detected in the interface of the trypsin digestion front. This ultrasound reflection signal was used to differentiate the deformations of different portions of the cartilage throughout its depth when a load was applied. The equilibrium compression moduli of the digested, undigested and entire portions of articular cartilage were measured. The modulus of the cartilage without any digestion was 660+/-230 kPa. After 1h digestion with 1 mg ml(-1) trypsin solution, the thickness of the digested portion was 0.50+/-0.06m, and the modulus of the entire cartilage layer changed to 125+/-42kPa. The moduli of the digested and undigested portions were 58+/-24 kPa and 470+/-31 kPa, respectively. Similar results were obtained for the cartilage with trypsin digestion for 2 h.
ISSN:0140-0118
1741-0444
DOI:10.1007/BF02345143