A Novel Form of Gonadotropin-Releasing Hormone in the Medaka, Oryzias latipes

The present study has identified three molecular forms of gonadotropin-releasing hormone (GnRH) in the brain of a teleost, the medaka, by isolation of their cDNAs. This species has a novel GnRH, which is here named medaka-type GnRH (mdGnRH), in addition to two characterized forms, chicken-II-type Gn...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-09, Vol.276 (1), p.298-303
Main Authors: Okubo, Kataaki, Amano, Masafumi, Yoshiura, Yasutoshi, Suetake, Hiroaki, Aida, Katsumi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Brain - metabolism
cGnRH-II
DNA, Complementary - analysis
DNA, Complementary - genetics
gene expression
gonadotropin-releasing hormone (GnRH)
Gonadotropin-Releasing Hormone - analysis
Gonadotropin-Releasing Hormone - genetics
Gonadotropin-Releasing Hormone - metabolism
In Situ Hybridization
mdGnRH
mdGnRH gene
medaka
mGnRH gene
molecular forms
Molecular Sequence Data
Oryzias
Oryzias latipes
Phylogeny
sbGnRH gene
sGnRH
sGnRH gene
Transcription, Genetic
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Summary:The present study has identified three molecular forms of gonadotropin-releasing hormone (GnRH) in the brain of a teleost, the medaka, by isolation of their cDNAs. This species has a novel GnRH, which is here named medaka-type GnRH (mdGnRH), in addition to two characterized forms, chicken-II-type GnRH (cGnRH-II) and salmon-type GnRH (sGnRH). Phylogenetic analysis showed that mdGnRH is a medaka homolog of and seabream-type GnRH (sbGnRH) and mammalian-type GnRH (mGnRH) in other species, and suggested that all vertebrates have three distinct GnRHs. Furthermore, in situ hybridization revealed that the mdGnRH gene is expressed only in neurons clustered within the preoptic area as sbGnRH and mGnRH genes in other species are, while the genes for cGnRH-II and sGnRH are only in the midbrain tegmentum and nucleus olfactoretinalis, respectively. This result suggested that mdGnRH is a hypophysiotropic factor and the other two forms are involved in other physiological events as neuromodulators or neurotransmitters.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.3476