Isolation of mitochondria from Plasmodium falciparum showing dihydroorotate dependent respiration

Using N 2 cavitation, we established a protocol to prepare the active mitochondria from Plasmodium falciparum showing a higher succinate dehydrogenase activity than previously reported and a dihydroorotate-dependent respiration. The fact that fumarate partially inhibited the dihydroorotate dependent...

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Bibliographic Details
Published in:Parasitology international 2001-11, Vol.50 (4), p.273-278
Main Authors: Takashima, Eizo, Takamiya, Shinzaburo, Takeo, Satoru, Mi-ichi, Fumika, Amino, Hisako, Kita, Kiyoshi
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Dihydroorotate oxidase
Fumarate reductase
Fumarates - metabolism
Mitochondria
Mitochondria - enzymology
Mitochondria - metabolism
Orotic Acid - analogs & derivatives
Orotic Acid - metabolism
Oxygen - metabolism
Oxygen Consumption
Plasmodium falciparum
Plasmodium falciparum - enzymology
Plasmodium falciparum - metabolism
quinol-fumarate reductase
Succinate dehydrogenase
Succinate Dehydrogenase - analysis
Succinate Dehydrogenase - metabolism
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Summary:Using N 2 cavitation, we established a protocol to prepare the active mitochondria from Plasmodium falciparum showing a higher succinate dehydrogenase activity than previously reported and a dihydroorotate-dependent respiration. The fact that fumarate partially inhibited the dihydroorotate dependent respiration suggests that complex II (succinate–ubiquinone reductase/quinol–fumarate reductase) in the erythrocytic stage cells of P. falciparum functions as a quinol–fumarate reductase.
ISSN:1383-5769
1873-0329
DOI:10.1016/S1383-5769(01)00085-X