AMP-Activated protein kinase is activated by the stimulations of G(q)-coupled receptors

• Published in: Biochemical and biophysical research communications 2000-09, Vol.276 (1), p.16-22
• Main Authors: Kishi, K, Yuasa, T, Minami, A, Yamada, M, Hagi, A, Hayashi, H, Kemp, B E, Witters, L A, Ebina, Y
• Format: Article
• Language: English
• Subjects: AMP-Activated Protein Kinases; Animals; Biological Transport; CHO Cells; Cricetinae; Enzyme Activation; Glucose - metabolism; Glucose Transporter Type 4; GTP-Binding Protein alpha Subunits, Gq-G11; Heterotrimeric GTP-Binding Proteins - metabolism; Monosaccharide Transport Proteins - metabolism; Multienzyme Complexes - metabolism; Muscle Proteins; Protein-Serine-Threonine Kinases - metabolism; Receptors, Adrenergic, alpha-1 - metabolism; Receptors, Bradykinin - metabolism; Signal Transduction
• ISSN: 0006-291X
• DOI: 10.1006/bbrc.2000.3417

The AMP-activated protein kinase (AMPK) functions as a metabolic sensor that monitors cellular AMP and ATP levels. Platelet-activating factor (PAF) activates endogeneous AMPKalpha1 in Chinese hamster ovary cells expressing the PAF receptor coupled with both G(i) and G(q), but its activity was not inhibited after treatment with islet-activating protein. Norepinephrine and bradykinin also activated AMPKalpha1 in cells expressing the G(q)-coupled alpha(1b)-adrenergic receptor and bradykinin receptor, respectively. Stimulations of the G(i)-coupled alpha(2A)-adrenergic receptor, fMet-Leu-Phe receptor, prostaglandin EP3alpha receptor, and G(s)-coupled beta(2)-adrenergic receptor did not activate AMPKalpha1. AMPKalpha1 thus is activated specifically by stimulation of G(q)-coupled receptors. G(q)-coupled receptors transmit the signal for GLUT4 translocation and glucose uptake through an insulin-independent pathway. However, direct activation of AMPKalpha1 with treatment of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside did not trigger GLUT4 translocation nor stimulate glucose uptake in our cells. Thus, activation of AMPKalpha1 via G(q) is not sufficient to trigger GLUT4 translocation or stimulate glucose uptake.