IFN-gamma affects homing of diabetogenic T cells

IFN-gamma is a cytokine with pleiotropic functions that participates in immune and autoimmune responses. The lack of IFN-gamma is known to delay the development of autoimmune diabetes in nonobese diabetic (NOD) mice. Splenocytes from diabetic NOD and IFN-gamma knockout (KO) NOD mice transfer diabete...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2001-12, Vol.167 (11), p.6637-6643
Main Authors: Savinov, A Y, Wong, F S, Chervonsky, A V
Format: Article
Language:English
Subjects:
Adoptive Transfer
Animals
Antigen Presentation - genetics
CD8 antigen
Cell Movement - immunology
Diabetes Mellitus, Type 1 - etiology
Diabetes Mellitus, Type 1 - immunology
Diabetes Mellitus, Type 1 - pathology
Female
g-Interferon
Injections, Intravenous
Interferon-gamma - biosynthesis
Interferon-gamma - deficiency
Interferon-gamma - genetics
Interferon-gamma - physiology
Islets of Langerhans - immunology
Islets of Langerhans - metabolism
Islets of Langerhans - pathology
Lymphocyte Transfusion
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred NOD
Mice, Knockout
Mice, SCID
Spleen - pathology
Spleen - transplantation
T-Lymphocyte Subsets - immunology
T-Lymphocyte Subsets - pathology
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Summary:IFN-gamma is a cytokine with pleiotropic functions that participates in immune and autoimmune responses. The lack of IFN-gamma is known to delay the development of autoimmune diabetes in nonobese diabetic (NOD) mice. Splenocytes from diabetic NOD and IFN-gamma knockout (KO) NOD mice transfer diabetes into NOD recipients equally well. However, adoptive transfer of diabetogenic T cells from NOD mice into NOD.IFN-gamma-KO or NOD mice lacking beta-chain of IFN-gamma receptor (NOD.IFN-gammaRbeta-KO) appeared to be much less efficient. We found that IFN-gamma influences the ability of diabetogenic cells to penetrate pancreatic islets. Tracing in vivo of insulin-specific CD8+ T cells has shown that homing of these cells to the islets of Langerhans was affected by the lack of IFN-gamma. While adhesion of insulin-specific CD8+ cells to microvasculature was normal, the diapedesis was significantly impaired. This effect was reversible by treatment of the animals with rIFN-gamma. Thus, IFN-gamma may, among other effects, influence immune and autoimmune responses by supporting the homing of activated T cells.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.167.11.6637