Cloning of Precursors for Two MIH/VIH-Related Peptides in the Prawn, Macrobrachium rosenbergii

Two cDNA clones (634 and 1366 bp) encoding MIH/VIH (molt-inhibiting hormone/vitellogenesis-inhibiting hormone)-related peptides were isolated and sequenced from a Macrobrachium rosenbergii eyestalk ganglia cDNA library. The clones contain a 360 and 339 bp open-reading frame, and their conceptually t...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-11, Vol.289 (2), p.407-413
Main Authors: Yang, Wei-Jun, Rao, K.Ranga
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Carrier Proteins - genetics
cDNA sequence
Cloning, Molecular
DNA, Complementary - metabolism
eyestalk ganglia
Female
Gene Library
Invertebrate Hormones
Macrobrachium rosenbergii
Male
MIH protein
Molecular Sequence Data
molt-inhibiting hormone
Neuropeptides - genetics
Palaemonidae
Peptides - chemistry
Poly A
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Tissue Distribution
Transcription, Genetic
VIH protein
vitellogenesis-inhibiting hormone
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two cDNA clones (634 and 1366 bp) encoding MIH/VIH (molt-inhibiting hormone/vitellogenesis-inhibiting hormone)-related peptides were isolated and sequenced from a Macrobrachium rosenbergii eyestalk ganglia cDNA library. The clones contain a 360 and 339 bp open-reading frame, and their conceptually translated peptides consist of a 41 and 34 amino acid signal peptide, respectively, and a 78 amino acid residue mature peptide hormone. The amino acid sequences of the peptides exhibit higher identities with other known MIHs and VIH (44–69%) than with CHHs (28–33%). This is the first report describing the cloning and sequencing of two MIH/VIH-related peptides in a single crustacean species. Transcription of these mRNAs was detected in the eyestalk ganglia, but not in the thoracic ganglia, hepatopancreas, gut, gill, heart, or muscle.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.6019