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Interaction of cyanine dyes with nucleic acids. Part 19: new method for the covalent labeling of oligonucleotides with pyrylium cyanine dyes
New chemistry for the fluorescent labeling of oligonucleotides with cyanine dyes is proposed. It is based on the use of pyrylium salts as amine-specific reagents. Monomethyne pyrylium cyanine dye 1 was covalently linked to 5′-aminoalkyl modified oligonucleotide, with simultaneous conversion of the n...
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| Published in: | Bioorganic & medicinal chemistry letters 2000-10, Vol.10 (19), p.2201-2204 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c390t-4f307c3eb2dcce9ecd6b610f48f40e0b8d29e51c0f487d844646d47491f4c0673 |
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| cites | cdi_FETCH-LOGICAL-c390t-4f307c3eb2dcce9ecd6b610f48f40e0b8d29e51c0f487d844646d47491f4c0673 |
| container_end_page | 2204 |
| container_issue | 19 |
| container_start_page | 2201 |
| container_title | Bioorganic & medicinal chemistry letters |
| container_volume | 10 |
| creator | Yarmoluk, Sergiy M Kostenko, Alexander M Dubey, Igor Y |
| description | New chemistry for the fluorescent labeling of oligonucleotides with cyanine dyes is proposed. It is based on the use of pyrylium salts as amine-specific reagents. Monomethyne pyrylium cyanine dye
1
was covalently linked to 5′-aminoalkyl modified oligonucleotide, with simultaneous conversion of the non-fluorescent dye
1
into fluorescent pyridinium cyanine structure
2
. |
| doi_str_mv | 10.1016/S0960-894X(00)00435-2 |
| format | article |
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1
was covalently linked to 5′-aminoalkyl modified oligonucleotide, with simultaneous conversion of the non-fluorescent dye
1
into fluorescent pyridinium cyanine structure
2
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| fulltext | fulltext |
| identifier | ISSN: 0960-894X |
| ispartof | Bioorganic & medicinal chemistry letters, 2000-10, Vol.10 (19), p.2201-2204 |
| issn | 0960-894X 1464-3405 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72296103 |
| source | ScienceDirect Journals |
| subjects | Amines - chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Fundamental and applied biological sciences. Psychology General aspects, investigation methods Molecular Structure Nucleic acids Oligonucleotides - chemistry Oligonucleotides - metabolism Staining and Labeling - methods Thiazoles - chemistry Thiazoles - metabolism |
| title | Interaction of cyanine dyes with nucleic acids. Part 19: new method for the covalent labeling of oligonucleotides with pyrylium cyanine dyes |
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