Induction of Apoptosis in Human Leukaemic Cell Lines K562, HL60 and U937 by Diethylhexylphthalate Isolated from Aloe vera Linne

We investigated the effect of diethylhexylphthalate (DEHP) from Aloe vera Linne on the apoptosis of human leukaemic cell lines K562, HL60 and U937 to examine its pharmacological activity. At a level of 10 μ mL−1 DEHP a significant anti‐leukaemic effect was observed for all three cell lines, as measu...

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Published in:Journal of pharmacy and pharmacology 2000-08, Vol.52 (8), p.1037-1041
Main Authors: LEE, KEYONG HO, HONG, HEE SUN, LEE, CHI HO, KIM, CHANG HAN
Format: Article
Language:English
Subjects:
Aloe
Apoptosis - drug effects
Biological and medical sciences
Diethylhexyl Phthalate - isolation & purification
Diethylhexyl Phthalate - pharmacology
Electrophoresis, Agar Gel
Flow Cytometry
General pharmacology
HL-60 Cells - drug effects
Humans
K562 Cells - drug effects
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plants, Medicinal
Tumor Cells, Cultured
Tumor Stem Cell Assay
U937 Cells - drug effects
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Summary:We investigated the effect of diethylhexylphthalate (DEHP) from Aloe vera Linne on the apoptosis of human leukaemic cell lines K562, HL60 and U937 to examine its pharmacological activity. At a level of 10 μ mL−1 DEHP a significant anti‐leukaemic effect was observed for all three cell lines, as measured by clonogenic assay. After treatment with 10 μg mL−1 DEHP for 4 h, agarose gel electrophoresis and flow cytometric analysis confirmed the occurrence of apoptosis. These results indicate that DEHP isolated from Aloe vera Linne has a potent anti‐leukaemic effect, and thus represents a new type of pharmacological activity with respect to human leukaemic cells.
ISSN:0022-3573
2042-7158
DOI:10.1211/0022357001774778