Inhibitory effects of morphine on some inflammation-related parameters in the goldfish Carassius auratus L

Acute peritonitis induced in the goldfish by intraperitoneal injection of a sterile Thioglycollate solution shows a typical pattern with intraperitoneal exudation of serum proteins followed by influx of leucocytes (mainly heterophils/macrophages) correlated with elevated levels of chemotactic factor...

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Bibliographic Details
Published in:Fish & shellfish immunology 2000-08, Vol.10 (6), p.531-542
Main Authors: Chadzinska, M, Scislowska-Czarnecka, A, Plytycz, B
Format: Article
Language:English
Subjects:
Analgesics, Opioid - administration & dosage
Analgesics, Opioid - immunology
Analgesics, Opioid - therapeutic use
Animals
Carassius auratus
Chemotaxis
Coloring Agents - chemistry
Evans Blue - chemistry
Exudates and Transudates
Fish Diseases - drug therapy
Fish Diseases - immunology
Goldfish - immunology
Injections, Intraperitoneal - veterinary
Leukocyte Count - veterinary
morphine
Morphine - administration & dosage
Morphine - immunology
Morphine - therapeutic use
Naltrexone - administration & dosage
Narcotic Antagonists - administration & dosage
Peritonitis - drug therapy
Peritonitis - immunology
Peritonitis - veterinary
Thioglycolates - administration & dosage
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Summary:Acute peritonitis induced in the goldfish by intraperitoneal injection of a sterile Thioglycollate solution shows a typical pattern with intraperitoneal exudation of serum proteins followed by influx of leucocytes (mainly heterophils/macrophages) correlated with elevated levels of chemotactic factors in peritoneal fluid and blood plasma. Supplementation of Thioglycollate with morphine (20 mg kg(-1) b.w.) does not affect the leakage of serum proteins into peritoneum. In contrast, it reduces the number of exudate peritoneal leucocytes (among them heterophils/macrophages) to the control level and decreases the level of peritoneal fluid/plasma chemoattractants, both effects being reversed by naltrexone pretreatment. Morphine itself acts as a chemokinetic factor for fish leucocytes as it increases their random movements. Therefore inhibitory effects of morphine on accumulation of exudate cells might be explained by inhibition of the production/release of chemotactic factors and/or reduced sensitivity of leucocytes to chemotactic signals. The effects of morphine on the goldfish peritonitis are in concordance with those described recently in Atlantic salmon and CB6 mice.
ISSN:1050-4648
DOI:10.1006/fsim.2000.0270