Renibacterium salmoninarum isolates from different sources possess two highly conserved copies of the rRNA operon

The nucleotide sequences of the rRNA genes and the 5' flanking region were determined for R. salmoninarum ATCC 33209T from overlapping products generated by PCR amplification from the genomic DNA. Comparison of the sequences with rRNA genes from a variety of bacteria demonstrated the close rela...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2000-07, Vol.78 (1), p.51-61
Main Authors: GRAYSON, T. Hilton, ALEXANDER, Sarah M, COOPER, Lynne F, GILPIN, Martyn L
Format: Article
Language:English
Subjects:
Actinomycetales - genetics
Bacteria
Bacteriology
Base Sequence
Biological and medical sciences
Conserved Sequence
Deoxyribonucleic acid
DNA
DNA, Ribosomal - genetics
Fundamental and applied biological sciences. Psychology
Gene Dosage
Genetic Variation
Genetics
Gram-Positive Asporogenous Rods, Regular - genetics
Microbiology
Molecular Sequence Data
Operon
Phylogeny
Polymerase Chain Reaction
Renibacterium salmoninarum
Restriction Mapping
RNA, Ribosomal - genetics
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 23S - genetics
RNA, Ribosomal, 5S - genetics
rRNA
rRNA operon
Sequence Analysis, DNA
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Summary:The nucleotide sequences of the rRNA genes and the 5' flanking region were determined for R. salmoninarum ATCC 33209T from overlapping products generated by PCR amplification from the genomic DNA. Comparison of the sequences with rRNA genes from a variety of bacteria demonstrated the close relatedness between R. salmoninarum and the high G+C group of the actinobacteria, in particular, Arthrobacter species. A regulatory element within the 5' leader of the rRNA operon was identical to an element, CL2, described for mycobacteria. PCR, DNA sequence analysis, and DNA hybridisation were performed to examine variation between isolates from diverse sources which represented the four 16S-23S rRNA intergenic spacer sequevars previously described for R. salmoninarum. Two 23S-5S rRNA intergenic spacer sequevars of identical length were found. DNA hybridisation using probes complementary to 23S rDNA and 16S rDNA identified two rRNA operons which were identical or nearly identical amongst 40 isolates sourced from a variety of countries.
ISSN:0003-6072
1572-9699
DOI:10.1023/A:1002745129625